重组人NGF蛋白(Active) (ab9796)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
描述
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产品名称
重组人NGF蛋白(Active)
参阅全部 NGF 蛋白酶 -
生物活性
Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
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纯度
>= 98 % SDS-PAGE.
>=98% HPLC analyses. Sterile filtered. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA -
预测分子量
27 kDa -
氨基酸
122 to 241 -
额外的序列信息
Full length mature protein, without the signal peptide or the propeptide. The functional form is a non-covalently linked homodimer 27 kDa.
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技术指标
Our Abpromise guarantee covers the use of ab9796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
HPLC
Functional Studies
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Please reconstitute this product to 0.1-1 mg/ml in water. Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA) for long term storage.
常规信息
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别名
- Beta nerve growth factor
- Beta NGF
- Beta-nerve growth factor
see all -
功能
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). -
疾病相关
Neuropathy, hereditary sensory and autonomic, 5 -
序列相似性
Belongs to the NGF-beta family. -
细胞定位
Secreted. - Information by UniProt
图片
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Western blot - Recombinant human NGF protein (ab9796)All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution
Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab9796 尚未被引用在任何文献中。
