重组人Neuroglobin蛋白(ab63278)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: WB, ELISA, SDS-PAGE
描述
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产品名称
重组人Neuroglobin蛋白 -
纯度
> 95 % SDS-PAGE.
Please filter the product by an appropriate sterile filter before using it in the cell culture. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
M-ERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG E
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技术指标
Our Abpromise guarantee covers the use of ab63278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
ELISA
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.134% PBS, 0.58% Sodium chloride
常规信息
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别名
- Neuroglobin
- NGB
- NGB_HUMAN
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功能
Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis. -
组织特异性
Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus. -
序列相似性
Belongs to the globin family. -
翻译后修饰
A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO.
Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity. -
细胞定位
Perikaryon. Cytoplasm. Mitochondrion. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human Neuroglobin protein (ab63278)LANE 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa. LANE 2. reduced and heated sample, 5µg/lane. LANE 3. non-reduced and non-heated sample, 5µg/lane.
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Western blot - Recombinant Human Neuroglobin protein (ab63278)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab63278 被引用在 2 文献中.
- Gao Y et al. Regulatory role of microRNA-320 during off-pump coronary artery bypass grafting with dexmedetomidine adjunct anesthesia. Exp Ther Med 22:1201 (2021). PubMed: 34584546
- Amri F et al. Neuroglobin protects astroglial cells from hydrogen peroxide-induced oxidative stress and apoptotic cell death. J Neurochem 140:151-169 (2017). PubMed: 27787886
