重组人NEK7蛋白(ab60703)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人NEK7蛋白
参阅全部 NEK7 蛋白酶 -
纯度
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
标签
GST tag N-Terminus
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
ab91092 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- EC 2.7.11.1
- NEK7
- NEK7_HUMAN
see all -
组织特异性
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. -
序列相似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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Functional Studies - Recombinant human NEK7 protein (ab60703)The specific activity of NEK7 (ab60703) was determined to be 9.2 nmol/min/mg as per activity assay protocol and was equivalent to 188 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human NEK7 protein (ab60703)SDS PAGE analysis of ab60703 -
Functional Studies - Recombinant human NEK7 protein (ab60703)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human NEK7 protein (ab60703)ab60703 on SDS-PAGE, MW ~63kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab60703 被引用在 1 文献中.
- Li Q et al. Licochalcone B specifically inhibits the NLRP3 inflammasome by disrupting NEK7-NLRP3 interaction. EMBO Rep 23:e53499 (2022). PubMed: 34882936