重组人NEDD8蛋白(ab185825)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, HPLC
描述
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产品名称
重组人NEDD8蛋白 -
纯度
> 95 % SDS-PAGE.
Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGHHHHHHGSLQDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIK KTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIE AHREQIGGMLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQR LIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG -
预测分子量
21 kDa including tags -
氨基酸
1 to 76 -
标签
His tag N-Terminus -
额外的序列信息
SUMO (Small Ubiquitin-like Modifier) tag
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab185825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
HPLC
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose -
复溶Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 3X PBS.
常规信息
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别名
- FLJ43224
- MGC104393
- MGC125896
see all -
功能
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. -
组织特异性
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. -
序列相似性
Belongs to the ubiquitin family. -
翻译后修饰
Cleavage of precursor form by UCHL3 or SENP8 is necessary for function. -
细胞定位
Nucleus. Mainly nuclear. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab185825 尚未被引用在任何文献中。