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重组人MTHFD1蛋白(ab116852)

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SDS-PAGE - Recombinant Human MTHFD1 protein (ab116852)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    描述

    • 产品名称

      重组人MTHFD1蛋白

    • 表达系统

      Wheat germ

    • Accession

      P11586

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSN LYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQ LPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCTPK GCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTA HLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGR KVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKP GKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGE TKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAH LYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAIT AANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRL GIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAP TEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPV SAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSII ADQIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVAT VRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGI PVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQ AVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAE VYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFL YPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

      • 预测分子量

        129 kDa including tags

      • 氨基酸

        1 to 935

    技术指标

    Our Abpromise guarantee covers the use of ab116852 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      ELISA

      SDS-PAGE

      Western blot

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    常规信息

    • 别名

      • 5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase
      • C 1 tetrahydrofolate synthase cytoplasmic
      • C1 tetrahydrofolate synthase cytoplasmic
      • C1 THF synthase
      • C1-THF synthase
      • C1TC
      • C1TC_HUMAN
      • Cytoplasmic C 1 tetrahydrofolate synthase
      • Formyltetrahydrofolate synthetase
      • Methenyltetrahydrofolate cyclohydrolase
      • Methylenetetrahydrofolate dehydrogenase
      • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase
      • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase
      • MTHFC
      • MTHFD
      • MTHFD1
      • NADP dependent cyclohydrolase formyltetrahydrofolate synthetase
      • OTTHUMP00000180657
      see all

    • 组织特异性

      Ubiquitous.

    • 通路

      One-carbon metabolism; tetrahydrofolate interconversion.

    • 疾病相关

      Defects in MTHFD1 may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTHFD1 may affect the risk of spina bifida via the maternal rather than the embryonic genotype.
      Genetic variation in MTHFD1 could be associated with susceptibility to colorectal cancer (CRC) [MIM:114500].

    • 序列相似性

      In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
      In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

    • 结构域

      This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

    • 细胞定位

      Cytoplasm.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human MTHFD1 protein (ab116852)
      SDS-PAGE - Recombinant Human MTHFD1 protein (ab116852)
      12.5% SDS-PAGE showing ab116852 at approximately 128.59kDa; stained with Coomassie Blue.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab116852？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab116852 尚未被引用在任何文献中。

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