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AB323114

Recombinant human MMRN1/ECM Protein Standard (His tag)

Recombinant human MMRN1/ECM Protein Standard (His tag)

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Recombinant human MMRN1/ECM Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.

查看别名

ECM, EMILIN4, GPIA*, MMRN, MMRN1, Multimerin-1, EMILIN-4, Elastin microfibril interface located protein 4, Endothelial cell multimerin, Elastin microfibril interfacer 4

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Sandwich ELISA - Recombinant human MMRN1/ECM Protein Standard (His tag) (AB323114)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant human MMRN1/ECM Protein Standard (His tag) (AB323114)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant human MMRN1/ECM Protein Standard (His tag) (AB323114)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MMRN1/ECM Protein Standard (His tag) (AB323114)

SDS-PAGE analysis of ab323114 under reducing conditions for 2ug protein.

关键信息

纯度

>80% SDS-PAGE

表达系统

HEK 293 cells

标签

His tag C-Terminus

应用

SDS-PAGE, sELISA

applications

生物活性

No

访问

Q13201

不含动物源

Yes

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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序列信息

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"59.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q13201","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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特殊说明

形式

Liquid

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常规信息

功能

Carrier protein for platelet (but not plasma) factor V/Va. Plays a role in the storage and stabilization of factor V in platelets. Upon release following platelet activation, may limit platelet and plasma factor Va-dependent thrombin generation. Ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, and may function as an extracellular matrix or adhesive protein.

翻译后修饰

The N-terminus is blocked.. Extensively N-glycosylated.. O-fucosylated within the EMI domain (at Thr-216 and Thr-265) by POFUT3 and POFUT4 (PubMed:39775168). O-fucosylation at Thr-216 and Thr-1055 are required for facilitating protein folding and secretion (PubMed:38237698, PubMed:39775168).

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产品实验方案

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靶点信息

Carrier protein for platelet (but not plasma) factor V/Va. Plays a role in the storage and stabilization of factor V in platelets. Upon release following platelet activation, may limit platelet and plasma factor Va-dependent thrombin generation. Ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, and may function as an extracellular matrix or adhesive protein.
See full target information MMRN1
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搭配使用产品

Reagents, Controls & Accessories

AB116028

Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (AB116028)

  • 5
  • 3

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