AB82955

Recombinant Human MMP9 protein (Proenzyme)

Name: 重组人MMP9蛋白(Proenzyme) (ab82955)| Abcam中文官网
Brand: Abcam Limited
SKU: AB82955
Price: 2935 CNY
Availability: InStock

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(2 Publications)

Recombinant Human MMP9 protein (Proenzyme) is a Human Fragment protein, in the 20 to 707 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, ELISA, WB.

查看别名

CLG4B, MMP9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB

5 Images

Sandwich ELISA - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

sELISA

Unknown

Sandwich ELISA - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

Standard curve for MMP9 (Analyte : ab82955); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [SB15c] to MMP9 (ab51203) at 5μg/ml and Detector Antibody Rabbit polyclonal to MMP9 - Hinge region (ab38902) at 0.5μg/ml.

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Western blot - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab73734 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406,

All lanes:

Western blot - Anti-MMP9 antibody (<a href='/products/primary-antibodies/mmp9-antibody-ab73734'>ab73734</a>) at 1 µg

All lanes:

Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 78 kDa

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Exposure time: 2min

Unknown

Western blot - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

All lanes:

Western blot - Anti-MMP9 antibody (<a href='/products/primary-antibodies/mmp9-antibody-ab73734'>ab73734</a>) at 1 µg

All lanes:

Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 78 kDa

true

Exposure time: 2min

Unknown

Western blot - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

Running buffer : MOPS. Conditions : Denatured/reduced. This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab76003 (rabbit-anti MMP9; 1.5 ug/mL) for 48 hours at 4°C. Before imaging, antibody binding was detected using infrared-labeled goat anti-rabbit (green) at 1 : 10,000 dilutions for 1 hour at room temperature.

All lanes:

Western blot - Anti-MMP9 antibody [EP1254] (<a href='/products/primary-antibodies/mmp9-antibody-ep1254-ab76003'>ab76003</a>) at 1.5 µg/mL

All lanes:

Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955) at 0.1 µg

Secondary

All lanes:

Goat anti-rabbit at 1/10000 dilution

Predicted band size: 78 kDa

Observed band size: 89 kDa

false

SDS-PAGE - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MMP9 protein (Proenzyme) (AB82955)

ab82955 on SDS-PAGE.

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

应用

SDS-PAGE, ELISA, WB

applications

生物活性

访问

P14780

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 7.2 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab82955 can be used as a WB positive control in conjunction with <a href='/products/primary-antibodies/mmp9-antibody-ab73734'>ab73734</a>." } } }

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序列信息

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":707,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P14780","tags":[{"tag":"6x His","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).

Biological function summary

Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.

Pathways

MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.

Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.

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特殊说明

形式

Liquid

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常规信息

功能

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed : 12879005, PubMed : 1480034, PubMed : 2551898). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed : 12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed : 32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed : 1480034). Degrades fibronectin but not laminin or Pz-peptide.

序列相似性

Belongs to the peptidase M10A family.

翻译后修饰

Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.. N- and O-glycosylated.

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information MMP9

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 19: PubMed29463024

2018

Overexpression of the Vitronectin V10 Subunit in Patients with Nonalcoholic Steatohepatitis: Implications for Noninvasive Diagnosis of NASH.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Del Ben,Diletta Overi,Licia Polimeni,Guido Carpino,Giancarlo Labbadia,Francesco Baratta,Daniele Pastori,Valeria Noce,Eugenio Gaudio,Francesco Angelico,Carmine Mancone

Biochimica et biophysica acta. Molecular basis of 1863:2808-2820 PubMed28712835

2017

Biological impact of advanced glycation endproducts on estrogen receptor-positive MCF-7 breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sabine Matou-Nasri,Hana Sharaf,Qiuyu Wang,Nasser Almobadel,Zaki Rabhan,Hamad Al-Eidi,Wesam Bin Yahya,Thadeo Trivilegio,Rizwan Ali,Nasser Al-Shanti,Nessar Ahmed

View all publications

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Recombinant Human MMP9 protein (Proenzyme)