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AB268790

重组人MGRN1蛋白(Active)

Recombinant human MGRN1 protein (Active)

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Recombinant human MGRN1 protein (Active) is a Human Fragment protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

查看别名

KIAA0544, RNF156, MGRN1, E3 ubiquitin-protein ligase MGRN1, Mahogunin RING finger protein 1, RING finger protein 156, RING-type E3 ubiquitin transferase MGRN1

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Functional Studies - Recombinant human MGRN1 protein (Active) (AB268790)
  • FuncS

Supplier Data

Functional Studies - Recombinant human MGRN1 protein (Active) (AB268790)

The specific activity of ab268790 was 1.4 nmol/min/mg in a ubiquitination assay using wild-type ubiquitin protein as substrate.

SDS-PAGE - Recombinant human MGRN1 protein (Active) (AB268790)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MGRN1 protein (Active) (AB268790)

SDS-PAGE analysis of ab268790.

关键信息

纯度

>90% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

The specific activity of ab268790 was 1.4 nmol/min/mg in a ubiquitination assay using wild-type ubiquitin protein as substrate.

访问

O60291

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"2-end","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O60291","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MGRN1 also known as Mahogunin RING finger 1 is a protein with a molecular mass of around 50 kDa. It is widely expressed in many tissues including the brain liver and kidneys. This protein participates in ubiquitination processes acting as an E3 ubiquitin-protein ligase. The function allows it to label proteins for degradation by the proteasome which is essential for maintaining cellular protein homeostasis.
Biological function summary

The E3 ubiquitin ligase activity of MGRN1 plays a significant role in cell cycle regulation and neuronal function. It exists as part of complexes that modulate pathways important for intracellular signaling. MGRN1 can influence the distribution and turnover of associated molecules within neurons impacting synaptic transmission and neuroplasticity. Through its regulation of the proteolytic pathways MGRN1 supports essential cellular activities.

Pathways

The ubiquitination process involving MGRN1 intersects with the Wnt signaling pathway and the Hedgehog signaling pathway. MGRN1 interacts with proteins like Dishevelled in the Wnt pathway which influences cell differentiation proliferation and apoptosis. In the Hedgehog signaling pathway it modulates stability and activity of pathway components coordinating developmental processes and tissue patterning.

Dysfunction of MGRN1 associates with neurodegenerative conditions and pigmentation disorders. Aberrations in its activity could potentially lead to the development of Parkinson's disease through interactions with proteins like parkin. Disruptions in MGRN1 function also affect pigment-related proteins contributing to conditions such as vitiligo an autoimmune disease that causes loss of skin color in patches. Understanding these connections helps in developing targeted therapeutic approaches.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H (PubMed : 17229889, PubMed : 19703557). Plays a role in the regulation of endosome-to-lysosome trafficking (PubMed : 17229889). Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production (PubMed : 19737927). Does not inhibit ADRB2-signaling (PubMed : 19737927). Does not promote MC1R ubiquitination (PubMed : 19737927). Acts also as a negative regulator of hedgehog signaling (By similarity).

翻译后修饰

Autoubiquitinated in vitro.

亚细胞定位

Early endosome

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产品实验方案

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靶点信息

E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H (PubMed : 17229889, PubMed : 19703557). Plays a role in the regulation of endosome-to-lysosome trafficking (PubMed : 17229889). Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production (PubMed : 19737927). Does not inhibit ADRB2-signaling (PubMed : 19737927). Does not promote MC1R ubiquitination (PubMed : 19737927). Acts also as a negative regulator of hedgehog signaling (By similarity).
See full target information MGRN1
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