重组人MERTK蛋白(Active) (ab271471)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Flow Cyt, SDS-PAGE
描述
-
产品名称
重组人MERTK蛋白(Active)
参阅全部 MERTK 蛋白酶 -
生物活性
Specific activity ≥50 pmol/min/µg.
Assay was done in kinase buffer containing 1 mM DTT using Poly- (Glu4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min.
-
纯度
>= 90 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
-
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
EDVVIDRNLL ILGKILGEGE FGSVMEGNLK QEDGTSLKVA VKTMKLDNSS QREIEEFLSE AACMKDFSHP NVIRLLGVCI EMSSQGIPKP MVILPFMKYG DLHTYLLYSR LETGPKHIPL QTLLKFMVDI ALGMEYLSNR NFLHRDLAAR NCMLRDDMTV CVADFGLSKK IYSGDYYRQG RIAKMPVKWI AIESLADRVY TSKSDVWAFG VTMWEIATRG MTPYPGVQNH EMYDYLLHGH RLKQPEDCLD ELYEIMYSCW RTDPLDRPTF SVLRLQLEKL LESLPDVRNQ ADVIY -
预测分子量
60 kDa including tags -
氨基酸
578 to 872 -
标签
GST tag N-Terminus -
额外的序列信息
Genbank: NM_006343
-
技术指标
Our Abpromise guarantee covers the use of ab271471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Flow Cytometry
SDS-PAGE
-
形式
Liquid -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.05% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- c MER
- c mer proto oncogene tyrosine kinase
- c-mer
see all -
功能
In case of filovirus infection, seems to function as a cell entry factor. -
组织特异性
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
疾病相关
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
图片
-
Functional Studies - Recombinant Human MERTK protein (Tagged) (ab271471)
Specific activity of ab271471.
Specific activity ≥50 pmol/min/µg.
Assay was done in kinase buffer containing 1 mM DTT using Poly- (Glu4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min.
-
SDS-PAGE - Recombinant Human MERTK protein (Tagged) (ab271471)SDS-PAGE analysis of ab271471.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab271471 尚未被引用在任何文献中。