AB131682

重组人 MCL1 蛋白 (His tag N-Terminus)

Name: 重组人MCL1蛋白 (ab131682)| Abcam中文官网
Brand: Abcam Limited
SKU: AB131682
Price: 5655 CNY
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Recombinant Human MCL1 protein (His tag N-Terminus)

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(2 Publications)

Recombinant Human MCL1 protein (His tag N-Terminus) is a Human Fragment protein, in the 1 to 327 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

查看别名

BCL2L3, MCL1, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, Bcl2-L-3

1 Images

SDS-PAGE - Recombinant Human MCL1 protein (His tag N-Terminus) (AB131682)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MCL1 protein (His tag N-Terminus) (AB131682)

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

应用

SDS-PAGE, Mass Spec

applications

生物活性

访问

Q07820

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKTINQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGG","proteinLength":"Fragment","predictedMolecularWeight":"37.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":327,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q07820","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCL1 also known as myeloid cell leukemia sequence 1 is an anti-apoptotic member of the BCL-2 family. This protein plays an important role in regulating cell survival by inhibiting apoptosis. MCL1 has a molecular weight of approximately 37 kDa. It is primarily expressed in the mitochondria and can be found in various tissues including heart liver placenta and kidneys.

Biological function summary

MCL1 protein functions to maintain cell viability by counteracting pro-apoptotic stimuli. It interacts within the cell as part of a complex network involving other BCL-2 family proteins. MCL1 binds and sequesters BH3-only proteins and Bax therefore preventing them from initiating the apoptotic cascade. This interaction helps cells survive under stress conditions by temporarily inhibiting apoptosis.

Pathways

MCL1 plays a significant role in the intrinsic (mitochondrial) apoptosis pathway and the cell signaling pathways that facilitate cell survival and proliferation. In the mitochondrial pathway it works closely with other BCL-2 family members like BCL-xL and BCL-2 itself to control the permeability of the mitochondrial outer membrane and thereby influence cytochrome c release. The PI3K/AKT pathway can also regulate MCL1 leading to increases in its expression and stability which promotes cell survival.

Researchers have linked alterations in MCL1 expression to various cancers including leukemia and lymphoma. Overexpression of MCL1 can contribute to chemoresistance in cancer cells by preventing apoptosis. This protein's function is also connected to cardiomyopathies where altered MCL1 expression in cardiac tissue can influence cell death and survival outcomes. BCL-2 and BCL-xL closely related proteins also implicate in these conditions highlighting the central role of the apoptotic regulatory network in disease development.

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特殊说明

形式

Liquid

附加说明

ab131682 was purified using conventional chromatography techniques.

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常规信息

功能

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

序列相似性

Belongs to the Bcl-2 family.

翻译后修饰

Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.. Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.. Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.. Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159 (PubMed:16543145). Deubiquitinated by USP20; leading to increased stability (PubMed:35063767).

亚细胞定位

Mitochondrion

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information MCL1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of medicinal chemistry 61:2962-2972 PubMed29584430

2018

Bicyclic Helical Peptides as Dual Inhibitors Selective for Bcl2A1 and Mcl-1 Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Aline D de Araujo,Junxian Lim,Kai-Chen Wu,Yibin Xiang,Andrew C Good,Renato Skerlj,David P Fairlie

Oncotarget 7:78958-78970 PubMed27738316

2016

Mitotic arrest-induced phosphorylation of Mcl-1 revisited using two-dimensional gel electrophoresis and phosphoproteomics: nine phosphorylation sites identified.

Applications

IP, WB

Species

Human, Human

Rong Chu,Sarah E Alford,Katherine Hart,Anisha Kothari,Samuel G Mackintosh,Matthew R Kovak,Timothy C Chambers

View all publications

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重组人 MCL1 蛋白 (His tag N-Terminus)

Recombinant Human MCL1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human MCL1 protein (His tag N-Terminus) (AB131682)

关键信息

纯度

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液