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重组人MBD4/MED1蛋白(ab126003)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Suitable for: SDS-PAGE

描述

  • 产品名称

    重组人MBD4/MED1蛋白

  • 纯度

    > 95 % SDS-PAGE.
    Purified via His tag

  • 表达系统

    Escherichia coli

  • Accession

    O95243

  • 蛋白长度

    Protein fragment

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 预测分子量

      26 kDa

    • 氨基酸

      68 to 300

技术指标

Our Abpromise guarantee covers the use of ab126003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 形式

    Lyophilized

  • 补充说明

     This product was previously labelled as MBD4

     

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • 复溶
    Reconstitute with water to desired concentration.

常规信息

  • 别名

    • 3 N(4) ethenocytosine glycosylase
    • G/5 fluorouracil mismatch glycosylase with biphasic kinetics
    • G/T mismatch glycosylase
    • G/U mismatch glycosylase
    • MBD 4
    • MBD4
    • MBD4_HUMAN
    • MED 1
    • MED1
    • Methyl CpG binding domain protein 4
    • Methyl CpG binding endonuclease 1
    • Methyl CpG binding protein MBD4
    • Methyl-CpG-binding domain protein 4
    • Methyl-CpG-binding endonuclease 1
    • Methyl-CpG-binding protein MBD4
    • Mismatch specific DNA N glycosylase
    • Mismatch-specific DNA N-glycosylase
    • Putative methyl CpG binding protein
    see all

  • 功能

    Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

  • 序列相似性

    Contains 1 MBD (methyl-CpG-binding) domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab126003？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab126003 尚未被引用在任何文献中。

客户评价及客户问答

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Question

Dear technical,

ab126003,ab4918
He knows those proteins are not guaranteed for ELISA but needs for his test with antibodies. I searched ab89534 and ab71007 for MBD1. Please let me know which one is better choice and give me information about MBD4 antibody. If you recommend one of antibody from your catalog, I'll introduce it with discount code.

Best regards

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Answer

Thank you for your inquiry.

MBD1 :

I would like to point out that ab4918 is 13 amino acid synthetic peptide whose sequences are derived from human MBD1 protein, C-R(593)SKDLKKPGARK(605) (amino to carboxyl terminus). This peptide can be used with studies using ab2846, but not with other antibodies since it is only a very short peptide.

ab71007 and ab89534 (both anti MBD1 antibodies cannot be used with ab4918. They will not bind this specific sequence.

Both these antibodies will work with a full length protein. Unfortunately, we do not currently offer this in our catalog.

MBD4 :

ab126003 is a recombinant fragment of Human MBD4 (amino acids 68-300) with a His-DHFR tag.
Please be aware thatab84754 is not compatible with ab126003.

I am happy to confirm that ab124460 (https://www.abcam.com/MBD4MED1-antibody-ab124460.html (or use the following: https://www.abcam.com/MBD4MED1-antibody-ab124460.html).)will bind ab126003 as it is the immunogen sequence.

Butab124460 is not tested for ELISA. I could offer your customer to take part in the testing discount program for this antibody in ELISA.

I am sorry that I did not have a 100% positive answer for your customer and hope this information is nevertheless helpful.

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