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AB275794

重组人MASPIN蛋白(His tag)

Recombinant Human MASPIN protein (His tag)

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Recombinant Human MASPIN protein (His tag) is a Human Full Length protein, in the 1 to 375 aa range, expressed in Escherichia coli, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

查看别名

PI5, SERPINB5, Serpin B5, Maspin, Peptidase inhibitor 5, PI-5

1 Images
SDS-PAGE - Recombinant Human MASPIN protein (His tag) (AB275794)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MASPIN protein (His tag) (AB275794)

SDS-PAGE analysis of ab275794.

关键信息

纯度

>98% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

P36952

不含动物源

No

不含载体蛋白

No

种属

Human

复溶

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

存储溶液

pH: 8 Preservative: 0.8% Proclin 300 Constituents: 79.82% Trehalose, 14.02% Sodium chloride, 3.86% Tris, 0.46% EDTA, 0.24% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.16% Sodium-N-Lauroylsarcosinate

storage-buffer

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搭配使用产品

Primary Antibodies

AB182785

Anti-MASPIN antibody

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASPIN antibody (AB182785)

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Assay Kits

AB238270

Mouse MASPIN ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse MASPIN ELISA Kit (AB238270)

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFSESETKECPFRVNKTDTKPVQMMNMEATFCMGNIDSINCKIIELPFQNKHLSMFILLPKDVEDESTGLEKIEKQLNSESLSQWTNPSTMANAKVKLSIPKFKVEKMIDPKACLENLGLKHIFSEDTSDFSGMSETKGVALSNVIHKVCLEITEDGGDSIEVPGARILQHKDELNADHPFIYIIRHNKTRNIIFFGKFCSP","proteinLength":"Full Length","predictedMolecularWeight":"45.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":375,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P36952","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Lyophilized
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MASPIN also known as SERPINB5 is a non-inhibitory serine protease inhibitor with a mass of approximately 42 kDa. It functions mechanically to regulate cell adhesion migration apoptosis and angiogenesis. MASPIN localizes mainly in the cytoplasm and nucleus and expresses predominantly in epithelial cells including those in the mammary gland prostate and keratinocytes in the skin. It acts as a tumor suppressor and researchers often use immunohistochemistry (IHC) to study its expression patterns in various tissues.
Biological function summary

MASPIN influences apoptosis and cell motility playing a critical role in tumor suppression. It does not form part of a complex but interacts directly with extracellular matrix components. MASPIN can inhibit angiogenesis by reducing the proliferation and migration of endothelial cells. Scientists have noted its role in maintaining normal cell architecture and inhibiting tumor cell invasion.

Pathways

Researchers place MASPIN in important signaling networks impacting cancer progression and metastasis. It participates in the PI3K/AKT pathway thereby influencing cell growth and survival. MASPIN also intersects with TGF-β signaling and related proteins like SMAD which mediate growth arrest and apoptosis. By altering these pathways MASPIN helps modulate responses to cellular stress and inhibit lethal growth in cancer cells.

MASPIN often associates with breast and prostate cancers. Its expression inversely correlates with tumor progression suggesting a protective role. MASPIN's interaction with proteins like p53 highlights its contribution to tumor suppression. Abnormal MASPIN presence links to aggressive disease phenotypes making it a potential biomarker for prognostic evaluation in oncology.
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常规信息

功能

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

序列相似性

Belongs to the serpin family. Ov-serpin subfamily.

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产品实验方案

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靶点信息

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
See full target information SERPINB5
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