AB316432

重组人MASP2蛋白Standard (His tag)

Name: 重组人MASP2蛋白Standard (His tag) (ab316432)| Abcam中文官网
Brand: Abcam Limited
SKU: AB316432
Price: 1406 CNY
Availability: InStock

Recombinant Human MASP2 Protein Standard (His tag)

Be the first to review this product! Submit a review

(0 Publication)

Recombinant Human MASP2 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.

查看别名

Mannan-binding lectin serine protease 2, MBL-associated serine protease 2, Mannose-binding protein-associated serine protease 2, MASP-2, MASP2

2 Images

Sandwich ELISA - Recombinant Human MASP2 Protein Standard (His tag) (AB316432)

sELISA

Supplier Data

Sandwich ELISA - Recombinant Human MASP2 Protein Standard (His tag) (AB316432)

Sandwich ELISA with the capture antibody dilution at 2 ug/mL and detector antibody dilution at 0.5 ug/mL.

SDS-PAGE - Recombinant Human MASP2 Protein Standard (His tag) (AB316432)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MASP2 Protein Standard (His tag) (AB316432)

SDS-PAGE analysis of ab316432

关键信息

纯度

>80% SDS-PAGE

表达系统

HEK 293 cells

应用

sELISA, SDS-PAGE

applications

生物活性

访问

O00187

不含动物源

Yes

不含载体蛋白

种属

Human

存储溶液

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

产品详情

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

style

left-column

序列信息

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"71.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O00187","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

style

left-column

性能和储存信息

运输条件

Dry Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MASP-2 also known as Mannan-binding lectin serine protease 2 is an important component of the innate immune system. This protein has a molecular mass of approximately 76 kDa and is expressed in the liver before being released into the bloodstream. MASP-2 primarily acts during the lectin pathway of the complement system where it plays an essential role in immune defense. The protein activates the complement cascade upon binding with carbohydrate molecules on pathogen surfaces contributing to opsonization and direct lysis of pathogens.

Biological function summary

MASP-2 associates with MBL (Mannose-binding lectin) and forms a complex critical for the lectin pathway's function. In this complex MASP-2 initiates the cleavage of complement proteins C4 and C2 forming the C3 convertase. This action enhances the immune response by promoting the clearance of pathogens. MASP-1 is another protease that works closely with MASP-2 in this pathway although the two have distinct roles—MASP-2 is primarily responsible for the activation of the downstream components of the cascade.

Pathways

MASP-2 plays an integral role in the complement system and particularly in the lectin pathway which is one of the three pathways of complement activation. It connects with both the classical and alternative pathways through the formation of C3 convertase a critical step in the complement cascade. The interactions with other proteins like MASP-1 and MBL are significant; MASP-1 has an additional role in the activation sequence by further augmenting MASP-2's activity and facilitating efficient pathogen clearance.

MASP-2 is connected to conditions like autoimmune diseases and recurrent infections due to its involvement in the complement system. For instance MASP-2 deficiency can lead to increased susceptibility to infections reflecting its role in pathogen clearance. The protein has been studied in relation to systemic lupus erythematosus where dysregulation of the complement pathway can exacerbate disease symptoms. Proteins such as MBL and MASP-1 which interact closely with MASP-2 also have roles in these disease states and further illustrate the importance of proper complement system functioning in disease prevention and control.

style

left-column

特殊说明

形式

Liquid

style

left-column

常规信息

功能

Precursor of a serum protease that activates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 11527969, PubMed : 22691502). The lectin complement system is activated following association of lectins, such as MBL2, FCN1, FCN2 or FCN3, to carbohydrates on the pathogen surface (PubMed : 22691502, PubMed : 22966085). MASP2 is cleaved and activated by MASP1 in response to lectin-binding to pathogen carbohydrates (PubMed : 10946292, PubMed : 22949645, PubMed : 22966085, PubMed : 9087411). Can activate prothrombin to thrombin (PubMed : 39924859).. Mannan-binding lectin serine protease 2 B chain. Serine protease component of the lectin complement pathway, which catalyzes cleavage and activation of C2 and C4, the next components of the complement pathway.

序列相似性

Belongs to the peptidase S1 family.

翻译后修饰

Activated by cleavage after Arg-444 by MASP1. The uncleaved zymogen is inactive towards synthetic substrates, but has sufficient activity to effect autocatalytic cleavage.. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

style

left-column

产品实验方案

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

See full target information Mannan-binding lectin serine protease 2 A chain

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组人MASP2蛋白Standard (His tag)

Recombinant Human MASP2 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human MASP2 Protein Standard (His tag) (AB316432)

SDS-PAGE - Recombinant Human MASP2 Protein Standard (His tag) (AB316432)

关键信息

纯度

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液