重组人Mark3蛋白(ab60012)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人Mark3蛋白 -
生物活性
Specific activity 722 nmol/min/mg.
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纯度
> 75 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- C-TAK1
- Cdc25C associated protein kinase 1
- Cdc25C-associated protein kinase 1
see all -
功能
Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. - Information by UniProt
图片
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Functional Studies - Recombinant human Mark3 protein (ab60012)The specific activity of Mark3 (ab60012) was determined to be 293.5 nmol/min/mg as per activity assay protocol and was equivalent to 830 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human Mark3 protein (ab60012)SDS PAGE analysis of ab60012 -
Functional Studies - Recombinant human Mark3 protein (ab60012)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human Mark3 protein (ab60012)ab60012 on SDS-PAGE. The extra bands are thought to be truncated MARK3 and tag.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60012 尚未被引用在任何文献中。