重组人MAP4K6蛋白(ab55712)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人MAP4K6蛋白 -
生物活性
Specific Activity: 19 nmol/min/mg
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纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
氨基酸
1 to 320
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab55712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- B55
- GCK family kinase MiNK
- hMINK
see all -
相关性
MAP4K6 is a serine/threonine kinase belonging to the germinal center kinase (GCK) family. These kinases regulate a wide variety of cellular processes by coupling cell surface receptors to MAPK pathways. This kinase appears to act upstream of the JUN N-terminal pathway and may be involved in brain development. It may also play a role in the response to environmental stress, in cytoskeleton reorganization, cell adhesion, and cell motility. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified. -
细胞定位
Cytoplasm. Cell junction; synapse; postsynaptic cell membrane; postsynaptic density. Cell projection; axon. Cell projection; dendrite. Isoform 4: Golgi apparatus
图片
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The specific activity of MAP4K6 (ab55712) was determined to be 22 nmol/min/mg as per activity assay protocol and was equivalent to 22 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab55712
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SDS-PAGE analysis of ab55712 with molecular weight markers. Approximate molecular weight 61kDa.
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Kinase assay using ab55712. Specific activity: 19 nmol/min/mg.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab55712 尚未被引用在任何文献中。