重组人MAP3K9蛋白(ab125554)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组人MAP3K9蛋白 -
生物活性
The specific activity of ab125554 was determined to be 116 nmol/min/mg.
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纯度
> 85 % Densitometry.
Purity was determined to be >85% by densitometry. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
77 kDa including tags -
氨基酸
1 to 433
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab125554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- M3K9_HUMAN
- Map3k9
- MEKK9
see all -
功能
Activates the JUN N-terminal pathway. -
组织特异性
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
翻译后修饰
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. - Information by UniProt
图片
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Functional Studies - Recombinant human MAP3K9 protein (ab125554)The specific activity of MAP3K9 (ab125554) was determined to be 133 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human MAP3K9 protein (ab125554)SDS PAGE analysis of ab125554 -
SDS-PAGE - Recombinant human MAP3K9 protein (ab125554)SDS PAGE analysis of ab125554 -
SDS-PAGE - Recombinant human MAP3K9 protein (ab125554)SDS-PAGE analysis of ab125554. -
Functional Studies - Recombinant human MAP3K9 protein (ab125554)The specific activity of ab125554 was determined to be 116 nmol/min/mg by Kinase Assay.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125554 尚未被引用在任何文献中。