重组人MAP3K8/COT蛋白(ab84728)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE
描述
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产品名称
重组人MAP3K8/COT蛋白
参阅全部 MAP3K8/COT 蛋白酶 -
纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
氨基酸
30 to 397 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab84728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
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形式
Liquid -
补充说明
This product was previously labelled as MAP3K8
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
常规信息
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别名
- AURA2
- c COT
- Cancer Osaka thyroid oncogene
see all -
功能
Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. -
组织特异性
Expressed in several normal tissues and human tumor-derived cell lines. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
发展阶段
Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle. -
翻译后修饰
Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human MAP3K8/COT protein (ab84728)ab84728 by SDS-Page
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab84728 尚未被引用在任何文献中。