重组人MAP3K8/COT蛋白(ab55700)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人MAP3K8/COT蛋白
参阅全部 MAP3K8/COT 蛋白酶 -
生物活性
Specific activity: 1012 nmol/min/mg
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纯度
> 75 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MENLYASEEP AVYEPSLMTM CQDSNQNDER SKSLLLSGQE VPWLSSVRYG TVEDLLAFAN HISNTAKHFY GQRPQESGIL LNMVITPQNG RYQIDSDVLL IPWKLTYRNI GSDFIPRGAF GKVYLAQDIK TKKRMACKLI PVDQFKPSDV EIQACFRHEN IAELYGAVLW GETVHLFMEA GEGGSVLEKL ESCGPMREFE IIWVTKHVLK GLDFLHSKKV IHHDIKPSNI VFMSTKAVLV DFGLSVQMTE DVYFPKDLRG TEIYMSPEVI LCRGHSTKAD IYSLGATLIH MQTGTPPWVK RYPRSAYPSY LYIIHKQAPP LEDIADDCSP GMRELIEASL ERNPNHRPRA ADLLKHEALN PPREDQPR -
预测分子量
70 kDa -
氨基酸
30 to 397 -
标签
GST tag N-Terminus
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab55700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- AURA2
- c COT
- Cancer Osaka thyroid oncogene
see all -
功能
Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. -
组织特异性
Expressed in several normal tissues and human tumor-derived cell lines. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
发展阶段
Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle. -
翻译后修饰
Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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Functional Studies - Recombinant human MAP3K8/COT protein (ab55700)The specific activity of MAP3K8/COT (ab55700) was determined to be 1100 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human MAP3K8/COT protein (ab55700)SDS PAGE analysis of ab55700 -
Functional Studies - Recombinant human MAP3K8/COT protein (ab55700)Kinase activity plot using ab55700. Specific activity: 1012 nmol/min/mg. -
SDS-PAGE - Recombinant human MAP3K8/COT protein (ab55700)SDS-PAGE analysis of ab55700, with molecular weight markers. Approximate molecular weight: 70kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab55700 尚未被引用在任何文献中。