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重组人MAP3K12蛋白(ab131688)

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SDS-PAGE - Recombinant Human MAP3K12 protein  (ab131688)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 75% SDS-PAGE
    • Suitable for: SDS-PAGE, WB

    描述

    • 产品名称

      重组人MAP3K12蛋白

    • 纯度

      > 75 % SDS-PAGE.
      Assessed by densitometry. Affinity purified.

    • 表达系统

      Baculovirus infected Sf9 cells

    • Accession

      Q9NS73

    • 蛋白长度

      Protein fragment

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 预测分子量

        94 kDa including tags

      • 氨基酸

        1 to 520

    技术指标

    Our Abpromise guarantee covers the use of ab131688 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      SDS-PAGE

      Western blot

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride

    常规信息

    • 别名

      • Dlk
      • Dual leucine zipper bearing kinase
      • Dual leucine zipper kinase DLK
      • Leucine-zipper protein kinase
      • M3K12_HUMAN
      • Map3k12
      • MAPK-upstream kinase
      • MEKK12
      • Mitogen-activated protein kinase kinase kinase 12
      • Mixed lineage kinase
      • MUK
      • Protein kinase MUK
      • ZPK
      • ZPKP1
      see all

    • 功能

      May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

    • 组织特异性

      Highly expressed in brain and kidney.

    • 序列相似性

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.

    • 结构域

      Interacts with MBIP through the leucine-zipper motif.

    • 翻译后修饰

      Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated.

    • 细胞定位

      Cytoplasm. Membrane.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human MAP3K12 protein  (ab131688)
      SDS-PAGE - Recombinant Human MAP3K12 protein  (ab131688)
      The purity of 131688 was determined to be >75% by densitometry.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab131688？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab131688 被引用在 1 文献中.

    • Cornett EM  et al. A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity. Sci Adv 4:eaav2623 (2018). PubMed: 30498785

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