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AB174389

重组人 MAP1D 蛋白 - BSA and Azide free (His tag N-Terminus)

Recombinant Human MAP1D protein - BSA and Azide free (His tag N-Terminus)

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Recombinant Human MAP1D protein - BSA and Azide free (His tag N-Terminus) is a Human Full Length protein, in the 20 to 335 aa range, expressed in Escherichia coli, with >85%, suitable for Mass Spec, SDS-PAGE.

查看别名

MAP1D, METAP1D, MAP 1D, MetAP 1D, Peptidase M 1D

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SDS-PAGE - Recombinant Human MAP1D protein - BSA and Azide free (His tag N-Terminus) (AB174389)
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Supplier Data

SDS-PAGE - Recombinant Human MAP1D protein - BSA and Azide free (His tag N-Terminus) (AB174389)

SDS-PAGE analysis MAP1D using 3 μg of ab174389 on a 15% gel.

关键信息

纯度

>85% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, Mass Spec

applications

生物活性

No

访问

Q6UB28

不含动物源

No

不含载体蛋白

Yes

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris-HCl buffer, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Use at 3 μg/lane</p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSSSPLNHIYLHKQSSSQQRRNFFFRRQRDISHSIVLPAAVSSAHPVPKHIKKPDYVTTGIVPDWGDSIEVKNEDQIQGLHQACQLARHVLLLAGKSLKVDMTTEEIDALVHREIISHNAYPSPLGYGGFPKSVCTSVNNVLCHGIPDSRPLQDGDIINIDVTVYYNGYHGDTSETFLVGNVDECGKKLVEVARRCRDEAIAACRAGAPFSVIGNTISHITHQNGFQVCPHFVGHGIGSYFHGHPEIWHHANDSDLPMEEGMAFTIEPIITEGSPEFKVLEDAWTVVSLDNQRSAQFEHTVLITSRGAQILTKLPHEA","proteinLength":"Full Length","predictedMolecularWeight":"37.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":335,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6UB28","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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特殊说明

形式

Liquid

附加说明

ab174389 is purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20 mM Tris pH 7.5, 2 mM EDTA.

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常规信息

功能

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed (By similarity). May play a role in colon tumorigenesis.

序列相似性

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.

亚细胞定位

Mitochondrion

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产品实验方案

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靶点信息

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed (By similarity). May play a role in colon tumorigenesis.
See full target information METAP1D
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