重组人M-CSF蛋白(Active) (ab62015)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
描述
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产品名称
重组人M-CSF蛋白(Active)
参阅全部 M-CSF 蛋白酶 -
生物活性
Method of Determination: NFS-60 cell proliferation
Acceptance Criteria: ED50 ≤10 ng/mL(≥ 1.0 x 10^5 units/mg)
The specific activity of Human M-CSF is 1.0 x 10^5 IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard.
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纯度
> 95 % SDS-PAGE. -
内毒素水平
<=1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGSM EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQG HERQSEGS -
预测分子量
37 kDa -
氨基酸
33 to 190
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技术指标
Our Abpromise guarantee covers the use of ab62015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at Room Temperature. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.16% Sodium phosphate, 0.58% Sodium chloride
Lyophilized from a sterile (0.2 micron) filtered aqueous solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Sterile water at 0.1 mg/mL Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
常规信息
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别名
- Colony stimulating factor 1
- Colony stimulating factor 1 (macrophage)
- Colony stimulating factor macrophage specific
see all -
功能
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. -
翻译后修饰
Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. -
细胞定位
Cell membrane and Secreted > extracellular space. - Information by UniProt
图片
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Functional Studies - Recombinant human M-CSF protein (ab62015)
ab62015 Induced Proliferation of NFS-60 Cells.
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SDS-PAGE - Recombinant human M-CSF protein (ab62015)1 ug of ab62015 on 4-20% Tris-Glycerine SDS-PAGE, stained with Coomassie Blue.
Lane 1: Non reducing
Lane 2: Reducing
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab62015 尚未被引用在任何文献中。