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AB62015

重组人M-CSF蛋白(Active)

Recombinant human M-CSF protein (Active)

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Recombinant human M-CSF protein (Active) is a Human Fragment protein, in the 33 to 190 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for FuncS, SDS-PAGE.

查看别名

Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1

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Functional Studies - Recombinant human M-CSF protein (Active) (AB62015)
  • FuncS

Supplier Data

Functional Studies - Recombinant human M-CSF protein (Active) (AB62015)

ab62015 Induced Proliferation of NFS-60 Cells.

SDS-PAGE - Recombinant human M-CSF protein (Active) (AB62015)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human M-CSF protein (Active) (AB62015)

1 ug of ab62015 on 4-20% Tris-Glycerine SDS-PAGE, stained with Coomassie Blue.

Lane 1 : Non reducing

Lane 2 : Reducing

关键信息

纯度

>95% SDS-PAGE

内毒素水平

<1 EU/µg

表达系统

Escherichia coli

标签

Tag free

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

Method of Determination: NFS-60 cell proliferation

Acceptance Criteria: ED50 ≤10 ng/mL(≥ 1.0 x 10^5 units/mg)

The specific activity of Human M-CSF is 1.0 x 10^5 IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard.

访问

P09603

不含动物源

No

不含载体蛋白

Yes

种属

Human

复溶

Reconstitute at 0.1 mg/mL in water

存储溶液

Constituents: 0.58% Sodium chloride, 0.16% Sodium phosphate

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Dimer: 37.1 kDa</p>" } } }
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序列信息

[{"sequence":"MEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQGHERQSEGSMEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQGHERQSEGS","proteinLength":"Fragment","predictedMolecularWeight":"37.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":190,"aminoAcidStart":33,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09603","tags":[]}]
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性能和储存信息

运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon reconstitution add a carrier protein (0.1% BSA)
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.
Biological function summary

M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.

Pathways

The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.

Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.
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特殊说明

形式

Lyophilized

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常规信息

功能

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

翻译后修饰

N-glycosylated.. Isoform 1. N-glycosylated.. Isoform 3. N-glycosylated.. O-glycosylated; contains chondroitin sulfate (PubMed:1531650, PubMed:8051056). O-glycosylated with core 1 or possibly core 8 glycans (PubMed:22171320, PubMed:23234360, PubMed:3264877).. Isoform 1. O-glycosylated.

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产品实验方案

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靶点信息

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
See full target information CSF1
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