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AB179494

重组人LTK蛋白

Recombinant human LTK protein

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Recombinant human LTK protein is a Human Fragment protein, in the 498 to 796 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

查看别名

TYK1, LTK, Leukocyte tyrosine kinase receptor, Protein tyrosine kinase 1

2 Images
Functional Studies - Recombinant human LTK protein (AB179494)
  • FuncS

Supplier Data

Functional Studies - Recombinant human LTK protein (AB179494)

The specific activity was determined to be 15 nmol/min/mg.

SDS-PAGE - Recombinant human LTK protein (AB179494)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human LTK protein (AB179494)

The purity was determined to be > 90% by densitometry. MW ~70 kDa.

关键信息

纯度

>90% Densitometry

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

FuncS, WB, SDS-PAGE

applications

生物活性

Yes

生物学活性

The specific activity was determined to be 15 nmol/min/mg.

访问

P29376

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

ab204848 (c Abl peptide) can be utilized as a substrate for assessing Kinase activity
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序列信息

[{"sequence":"LPPGVTEVSPANVTLLRALGHGAFGEVYEGLVIGLPGDSSPLQVAIKTLPELCSPQDELDFLMEALIISKFRHQNIVRCVGLSLRATPRLILLELMSGGDMKSFLRHSRPHLGQPSPLVMRDLLQLAQDIAQGCHYLEENHFIHRDIAARNCLLSCAGPSRVAKIGDFGMARDIYRASYYRRGDRALLPVKWMPPEAFLEGIFTSKTDSWSFGVLLWEIFSLGYMPYPGRTNQEVLDFVVGGGRMDPPRGCPGPVYRIMTQCWQHEPELRPSFASILERLQYCTQDPDVLNSLLPMELG","proteinLength":"Fragment","predictedMolecularWeight":"70 kDa","actualMolecularWeight":null,"aminoAcidEnd":796,"aminoAcidStart":498,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P29376","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Leukocyte Tyrosine Kinase (LTK) also known as ALK-like receptor tyrosine kinase is a receptor tyrosine kinase with a molecular weight of approximately 94 kDa. It primarily gets expressed in immune-related tissues such as the thymus and spleen as well as in the nervous system. LTK consists of an extracellular ligand-binding domain a single transmembrane domain and an intracellular kinase domain. It plays role in signal transduction processes by transferring a phosphate group to tyrosine residues on target proteins which initiates downstream signaling pathways.
Biological function summary

The LTK protein functions in the differentiation and development of cells particularly in the nervous and immune systems. It oligomerizes forming homodimers or heterodimers which activates its kinase activity. This activation promotes phosphorylation events that influence cellular processes like growth and survival. The receptor is not part of a larger complex but interacts directly with several adaptor proteins to exert its effects.

Pathways

LTK is involved in the MAPK/ERK and PI3K/AKT pathways important for cell growth and survival signaling. The MAPK/ERK pathway includes related proteins such as RAS and RAF while the PI3K/AKT pathway interacts with proteins like PIK3CA and AKT1. These pathways converge on transcription factors and regulators that ultimately coordinate cellular responses to external signals.

LTK shows involvement in the pathogenesis of certain types of cancer and neurodevelopmental disorders like neuroblastoma and developmental delay. In neuroblastoma aberrant expression or mutations in LTK link to tumorigenic processes. It shares a connection with the ALK protein another receptor tyrosine kinase which also exhibits alterations in various cancers. Understanding these associations helps in identifying potential therapeutic targets for these conditions.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Receptor with a tyrosine-protein kinase activity (PubMed : 10445845, PubMed : 20548102, PubMed : 30061385). Following activation by ALKAL1 or ALKAL2 ligands at the cell surface, transduces an extracellular signal into an intracellular response (PubMed : 30061385, PubMed : 34646012). Ligand-binding to the extracellular domain induces tyrosine kinase activation, leading to activation of the mitogen-activated protein kinase (MAPK) pathway (PubMed : 20548102). Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif (By similarity). The exact function of this protein is not known; studies with chimeric proteins demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival (PubMed : 18849880, PubMed : 9223670). Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport (PubMed : 20548102).

序列相似性

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

翻译后修饰

Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.

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产品实验方案

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靶点信息

Receptor with a tyrosine-protein kinase activity (PubMed : 10445845, PubMed : 20548102, PubMed : 30061385). Following activation by ALKAL1 or ALKAL2 ligands at the cell surface, transduces an extracellular signal into an intracellular response (PubMed : 30061385, PubMed : 34646012). Ligand-binding to the extracellular domain induces tyrosine kinase activation, leading to activation of the mitogen-activated protein kinase (MAPK) pathway (PubMed : 20548102). Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif (By similarity). The exact function of this protein is not known; studies with chimeric proteins demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival (PubMed : 18849880, PubMed : 9223670). Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport (PubMed : 20548102).
See full target information LTK
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