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重组人LRRK2蛋白(ab177261)

Submit a review Submit a question References (1)
Functional Studies - Recombinant human LRRK2 protein (ab177261)
  • SDS-PAGE - Recombinant human LRRK2 protein (ab177261)
  • SDS-PAGE - Recombinant human LRRK2 protein (ab177261)
  • Functional Studies - Recombinant human LRRK2 protein (ab177261)
  • SDS-PAGE - Recombinant human LRRK2 protein (ab177261)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 70% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

描述

  • 产品名称

    重组人LRRK2蛋白
    参阅全部 LRRK2 蛋白酶

  • 生物活性

    6 nmol/min/mg

  • 纯度

    > 70 % Densitometry.
    Affinity purified.

  • 表达系统

    Baculovirus infected Sf9 cells

  • Accession

    Q5S007

  • 蛋白长度

    Protein fragment

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 序列

      MRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLE LHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSR NDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGN KISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAA MPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHW KSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCL ENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKD IIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSA TVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLA VYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMS KITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQL VVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDEN ELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEG CPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYL LVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISP YMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITV PSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFN DGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRN IMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLL RQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLT RTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIAD YGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDIL TTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKE NPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASI WLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVS GTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGT ADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWG GCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAK QNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTL CLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLK NVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKEL AEKMRRTSVE

    • 预测分子量

      210 kDa including tags

    • 氨基酸

      968 to 2527

    • 标签

      GST tag N-Terminus

相关产品

技术指标

Our Abpromise guarantee covers the use of ab177261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

  • 形式

    Liquid

  • 补充说明

    It is recommended to store this protein at -80°C in glycerol to preserve the activity of the protein.

     

    ab204865 (Ezrin / Radixin / Moesin peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.005% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名

    • augmented in rheumatoid arthritis 17
    • AURA17
    • Dardarin
    • Leucine rich repeat kinase 2
    • leucine rich repeat serine threonine protein kinase 2
    • Leucine-rich repeat serine/threonine-protein kinase 2
    • LRRK 2
    • LRRK2
    • LRRK2_HUMAN
    • PARK 8
    • PARK8
    • RIPK7
    • ROCO 2
    • ROCO2
    see all

  • 功能

    Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.

  • 组织特异性

    Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.

  • 疾病相关

    Parkinson disease 8

  • 序列相似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 protein kinase domain.
    Contains 1 Roc domain.
    Contains 7 WD repeats.

  • 结构域

    The seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins.
    The Roc domain mediates homodimerization and regulates kinase activity.

  • 翻译后修饰

    Autophosphorylated.

  • 细胞定位

    Membrane. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane. Mitochondrion inner membrane. Mitochondrion matrix. Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes.
  • Information by UniProt

图片

  • Functional Studies - Recombinant human LRRK2 protein (ab177261)
    Functional Studies - Recombinant human LRRK2 protein (ab177261)
    The specific activity of LRRK2 (ab177261) was determined to be 5.8 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human LRRK2 protein (ab177261)
    SDS-PAGE - Recombinant human LRRK2 protein (ab177261)
    SDS PAGE analysis of ab177261
  • SDS-PAGE - Recombinant human LRRK2 protein (ab177261)
    SDS-PAGE - Recombinant human LRRK2 protein (ab177261)
    SDS PAGE analysis of ab177261
  • Functional Studies - Recombinant human LRRK2 protein (ab177261)
    Functional Studies - Recombinant human LRRK2 protein (ab177261)

    The specific activity of ab177261 was determined to be 6 nmol/min/mg.

  • SDS-PAGE - Recombinant human LRRK2 protein (ab177261)
    SDS-PAGE - Recombinant human LRRK2 protein (ab177261)

    SDS-PAGE analysis of ab177261.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab177261？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab177261 被引用在 1 文献中.

  • Yuan Y  et al. Single molecule array measures of LRRK2 kinase activity in serum link Parkinson's disease severity to peripheral inflammation. Mol Neurodegener 19:47 (2024). PubMed: 38862989

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