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AB115504

重组人Lipoprotein lipase

Recombinant Human Lipoprotein lipase

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Recombinant Human Lipoprotein lipase is a Human Full Length protein, in the 28 to 475 aa range, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, WB.

查看别名

LIPD, LPL, Lipoprotein lipase, Phospholipase A1

1 Images
SDS-PAGE - Recombinant Human Lipoprotein lipase (AB115504)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Lipoprotein lipase (AB115504)

14% SDS-PAGE showing ab115504
Lane 1 : M.W. marker – 14, 21, 31, 45, 66, 97 kDa
Lane 2 : reduced and boiled sample, 5μg/lane.
Lane 3 : non-reduced and non-boiled sample, 5μg/lane.

关键信息

纯度

>80% Densitometry

表达系统

HEK 293 cells

标签

DDDDK tag N-Terminus

应用

SDS-PAGE, WB

applications

生物活性

No

访问

P06858

不含动物源

No

不含载体蛋白

No

种属

Human

复溶

Reconstitute at 0.5 mg/mL in water

存储溶液

pH: 7.5 Constituents: 0.29% Sodium chloride, 0.24% Tris buffer

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"HVDYKDDDDKPAGADQRRDFIDIESKFALRTPEDTAEDTCHLIPGVAESVATCHFNHSSKTFMVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWLSRAQEHYPVSAGYTKLVGQDVARFINWMEEEFNYPLDNVHLLGYSLGAHAAGIAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTRGSPGRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLVKCSHERSIHLFIDSLLNEENPSKAYRCSSKEAFEKGLCLSCRKNRCNNLGYEISKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFSGTESETHTNQAFEISLYGTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMLKLKWKSDSYFSWSDWWSSPGFAIQKIRVKAGETQKKVIFCSREKVSHLQKGKAPAVFVKCHDKSLNKKSG","proteinLength":"Full Length","predictedMolecularWeight":"51.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":475,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P06858","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
False
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特殊说明

形式

Lyophilized

附加说明

ab115504 is filtered (0.4 µm).

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常规信息

功能

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).

序列相似性

Belongs to the AB hydrolase superfamily. Lipase family.

翻译后修饰

Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.

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产品实验方案

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靶点信息

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).
See full target information LPL
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搭配使用产品

Assay Kits

AB204721

Lipoprotein Lipase Assay Kit (Fluorometric)

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

  • 0
  • 0
Primary Antibodies

AB21356

Anti-Lipoprotein lipase antibody [LPL.A4]

Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

  • 4
  • 8
Assay Kits

AB102524

Lipase Assay Kit (Colorimetric)

Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)

  • 0
  • 0
Proteins & Peptides

AB226233

Recombinant Human Adipose Triglyceride Lipase protein (His tag)

SDS-PAGE - Recombinant Human Adipose Triglyceride Lipase protein (His tag) (AB226233)

  • 0
  • 0

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