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AB207944

重组 human LIMK2 protein (GST tag N-Terminus)

Recombinant human LIMK2 protein (GST tag N-Terminus)

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Recombinant human LIMK2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 638 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.

查看别名

LIM domain kinase 2, LIMK-2, LIMK2

2 Images
Functional Studies - Recombinant human LIMK2 protein (GST tag N-Terminus) (AB207944)
  • FuncS

Supplier Data

Functional Studies - Recombinant human LIMK2 protein (GST tag N-Terminus) (AB207944)

Sample Kinase Activity assay plot using ab207944, showing the specific activity to be 3 nmol/min/mg.

SDS-PAGE - Recombinant human LIMK2 protein (GST tag N-Terminus) (AB207944)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human LIMK2 protein (GST tag N-Terminus) (AB207944)

SDS-PAGE analysis of ab207944.

关键信息

纯度

>85% Densitometry

Affinity purified.

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

The specific activity of ab207944 was found to be 3 nmol/min/mg.

访问

P53671

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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搭配使用产品

Proteins & Peptides

AB62958

Recombinant Human Cofilin protein (His tag N-Terminus)

Western blot - Recombinant Human Cofilin protein (His tag N-Terminus) (AB62958)

  • 0
  • 0
Proteins & Peptides

AB314693

Recombinant Mouse Fgf8 Protein

HPLC - Recombinant Mouse Fgf8 Protein (AB314693)

  • 0
  • 0
Proteins & Peptides

AB70186

Recombinant Human LIM Kinase 1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human LIM Kinase 1 protein (His tag N-Terminus) (AB70186)

  • 0
  • 0
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Previously labelled as LIM kinase 2.
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序列信息

[{"linker":null,"sequence":"MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP","proteinLength":"Full Length","predictedMolecularWeight":"96 kDa","actualMolecularWeight":null,"aminoAcidEnd":638,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P53671","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LIMK2 also known as LIM domain kinase 2 is a protein that functions primarily as a serine/threonine kinase. This protein with a molecular weight of approximately 72 kDa plays an important role in regulating the cytoskeleton dynamics by phosphorylating and inactivating cofilin thereby affecting actin filament stability. LIMK2 is ubiquitously expressed with higher expression levels observed in the brain and certain types of cancer tissues indicating its importance in both normal and pathological cell processes.
Biological function summary

LIMK2 influences cell motility differentiation and division by modulating the actin cytoskeleton. It is a central component in various signaling complexes where it interacts with proteins such as ROCK and PAK which are upstream activators. By controlling actin filament assembly and disassembly LIMK2 impacts essential cellular functions including migration and invasion highlighting its potential role in cancer metastasis.

Pathways

LIMK2 integrates into signaling cascades linked to the Rho family of GTPases and the TGF-beta pathways. In these pathways it collaborates with proteins like ROCK to influence cytoskeletal reorganization. LIMK2's activity in these pathways emphasizes its role in maintaining cellular integrity and responding to extracellular stimuli which are critical components of cell cycle regulation and morphological changes.

Aberrant LIMK2 activity has connections with cancer progression and neurological disorders. In cancer upregulation can lead to increased tumor cell invasion and metastasis through its interaction with cofilin and the subsequent increase in actin filament assembly. In neurological contexts altered LIMK2 function associates with neurodegenerative diseases possibly due to its role in neuronal signaling networks and cytoskeletal dynamics. Understanding LIMK2’s interactions with proteins like cofilin offers insight into potential therapeutic targets for managing these diseases.
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常规信息

功能

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed : 10436159, PubMed : 11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed : 10436159, PubMed : 11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed : 22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed : 8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed : 25849865).

序列相似性

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

翻译后修饰

Phosphorylated on serine and/or threonine residues by ROCK1.

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产品实验方案

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靶点信息

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed : 10436159, PubMed : 11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed : 10436159, PubMed : 11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed : 22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed : 8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed : 25849865).
See full target information LIMK2
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