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重组人Laminin 2 alpha蛋白(ab114155)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human Laminin 2 alpha protein (ab114155)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: WB, ELISA, SDS-PAGE

    描述

    • 产品名称

      重组人Laminin 2 alpha蛋白

    • 表达系统

      Wheat germ

    • Accession

      P24043

    • 蛋白长度

      Protein fragment

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        DAGVPGHLCDGQWHKVTANKIKHRIELTVDGNQVEAQSPNPASTSADTND PVFVGGFPDDLKQFGLTTSIPFRGCIRSLKLTKGTGKPLEVNFAKALELR GVQPVSCPAN

      • 预测分子量

        38 kDa including tags

      • 氨基酸

        3013 to 3122

      • 标签

        GST tag N-Terminus

    技术指标

    Our Abpromise guarantee covers the use of ab114155 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Western blot

      ELISA

      SDS-PAGE

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    常规信息

    • 别名

      • LAMA 2
      • LAMA2
      • LAMA2_HUMAN
      • Laminin alpha 2
      • Laminin alpha 2 (merosin congenital muscular dystrophy)
      • Laminin alpha 2 chain
      • Laminin alpha 2 subunit
      • Laminin M
      • Laminin M chain
      • Laminin subunit alpha-2
      • Laminin-12 subunit alpha
      • Laminin-2 subunit alpha
      • Laminin-4 subunit alpha
      • LAMM
      • Merosin heavy chain
      see all

    • 功能

      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

    • 组织特异性

      Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.

    • 疾病相关

      Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.

    • 序列相似性

      Contains 17 laminin EGF-like domains.
      Contains 5 laminin G-like domains.
      Contains 2 laminin IV type A domains.
      Contains 1 laminin N-terminal domain.

    • 结构域

      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI, IV and G are globular.

    • 细胞定位

      Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human Laminin 2 alpha protein (ab114155)
      SDS-PAGE - Recombinant Human Laminin 2 alpha protein (ab114155)
      12.5% SDS-PAGE analysis of ab114155, stained with Coomassie Blue.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab114155？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab114155 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    Aim : The purpose of this protein to use it for phage display tech to select the antibodies and to make fusion protein. Also I will use this for other function assay like western etc.,

    Please advise me out of all laminin protein products that you have which one will serve best for my project.

    I would like to know information about ab114155

    1) Is the ab114155 product suits to my requirements ? if yes
    2) Is this is full length laminin 2 protein ?
    3) Is this has sequence for Laminin 2 alpha G domain ? All the 5 G domains are in Sequence?

    Read More
    Answer

    The human Laminin 2 alpha protein fragment ab114155 is not the full-length protein. It is a fragment, from amino acids 3013 - 3122 of the sequence given at the UniProt database entry for human Laminin subunit alpha-2, http://www.uniprot.org/uniprot/P24043 .

    DAGVPGHLCDGQWHKVTANKIKHRIELTVDGNQVEAQSPN PASTSADTNDPVFVGGFPDDLKQFGLTTSIPFRGCIRSLK LTKGTGKPLEVNFAKALELRGVQPVSCPAN

    This sequence contains the Laminin G-like 5 domain, which is aa 2939 – 3110, according to the UniProt sequence annotations.

    The fragment should be capable of selecting antibodies that recognize sequences with the range of amino acids 3013 - 3122. However, it has not, to our knowledge, been tested in this application.

    Read More

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