Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
产品名称重组人Lactate Dehydrogenase B/LDH-B蛋白
参阅全部 Lactate Dehydrogenase B/LDH-B 蛋白酶
Specific activity is > 300 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.
- Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH7.5 at 37°C, and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin.
- Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
- Add 50ul of recombinant LDHB protein in various concentrations (0.1ug, 0.5ug) in assay buffer.
- Mix by inversion and record the increase at A340nm for 5 minutes.
纯度> 95 % SDS-PAGE.
ab96765 is purified by using conventional chromatography techniques.
蛋白长度Full length protein
序列MGSSHHHHHH SSGLVPRGSHMATLKEKLIAPVAEEEATVP NNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGS LFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKF IIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSAR FRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMG TDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSR IHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKS ADTLWDIQKDLKDL
预测分子量37 kDa including tags
氨基酸1 to 334
标签His tag N-Terminus
Our Abpromise guarantee covers the use of ab96765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Previously labelled as Lactate Dehydrogenase B.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Epididymis secretory protein Li 281
- HEL S 281
- L lactate dehydrogenase B chain
通路Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
疾病相关Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
序列相似性Belongs to the LDH/MDH superfamily. LDH family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.