重组人Lactate Dehydrogenase B/LDH-B蛋白(ab96765)

Specific activity is > 300 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.

Activity Assay

1. Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH7.5 at 37°C, and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHB protein in various concentrations (0.1ug, 0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes.

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol

This product is an active protein and may elicit a biological response in vivo, handle with caution.