重组人L-Kynurenine Hydrolase蛋白Standard (His tag) (ab318259)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: Sandwich ELISA, SDS-PAGE
描述
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产品名称
重组人L-Kynurenine Hydrolase蛋白Standard (His tag)
参阅全部 L-Kynurenine Hydrolase 蛋白酶 -
纯度
>= 80 % SDS-PAGE.
His tag affinity purified -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
Yes -
性质
Recombinant -
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种属
Human -
序列
MEPSSLELPADTVQRIAAELKCHPTDERVALHLDEEDKLRHFRECFYIPK IQDLPPVDLSLVNKDENAIYFLGNSLGLQPKMVKTYLEEELDKWAKIAAY GHEVGKRPWITGDESIVGLMKDIVGANEKEIALMNALTVNLHLLMLSFFK PTPKRYKILLEAKAFPSDHYAIESQLQLHGLNIEESMRMIKPREGEETLR IEDILEVIEKEGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFD LAHAVGNVELYLHDWGVDFACWCSYKYLNAGAGGIAGAFIHEKHAHTIKP AL -
预测分子量
57 kDa including tags -
氨基酸
1 to 302 -
标签
His tag N-Terminus -
额外的序列信息
Intracellular
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相关产品
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Corresponding Antibody
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Corresponding kit
技术指标
Our Abpromise guarantee covers the use of ab318259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Sandwich ELISA
SDS-PAGE
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形式
Liquid -
补充说明
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Information available upon request.
常规信息
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别名
- kynU
- KYNU_HUMAN
- Kynureninase
see all -
功能
Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. -
组织特异性
Expressed in all tissues tested (heart, brain placenta, lung, liver, skeletal muscle, kidney and pancreas). Highest levels found in placenta, liver and lung. Expressed in all brain regions. -
通路
Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.
Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3. -
疾病相关
Note=Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. -
序列相似性
Belongs to the kynureninase family. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human L-Kynurenine Hydrolase Protein Standard (His tag) (ab318259)
SDS-PAGE analysis of AB318259 under reducing conditions for 2ug protein.
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Sandwich ELISA - Recombinant Human L-Kynurenine Hydrolase Protein Standard (His tag) (ab318259)Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab318259 尚未被引用在任何文献中。
