重组人L-Kynurenine Hydrolase蛋白(Active) (ab271581)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 85% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人L-Kynurenine Hydrolase蛋白(Active)
参阅全部 L-Kynurenine Hydrolase 蛋白酶 -
生物活性
Specific Activity: ≥75 pmol/min/µg. -
纯度
>= 85 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
EPSSLELPADTVQRIAAELKCHPTDERVALHLDEEDKLRHFRECFYIPKI QDLPPVDLSLVNKDENAIYFLGNSLGLQPKMVKTYLEEELDKWAKIAAYG HEVGKRPWITGDESIVGLMKDIVGANEKEIALMNALTVNLHLLMLSFFKP TPKRYKILLEAKAFPSDHYAIESQLQLHGLNIEESMRMIKPREGEETLRI EDILEVIEKEGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFDL AHAVGNVELYLHDWGVDFACWCSYKYLNAGAGGIAGAFIHEKHAHTIKPA LVGWFGHELSTRFKMDNKLQLIPGVCGFRISNPPILLVCSLHASLEIFKQ ATMKALRKKSVLLTGYLEYLIKHNYGKDKAATKKPVVNIITPSHVEERGC QLTITFSVPNKDVFQELEKRGVVCDKRNPNGIRVAPVPLYNSFHDVYKFT NLLTSILDSAETKN -
预测分子量
53 kDa -
氨基酸
1 to 465 -
标签
His tag C-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab271581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.53% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.00005% PLP, 0.00003% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- kynU
- KYNU_HUMAN
- Kynureninase
see all -
功能
Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. -
组织特异性
Expressed in all tissues tested (heart, brain placenta, lung, liver, skeletal muscle, kidney and pancreas). Highest levels found in placenta, liver and lung. Expressed in all brain regions. -
通路
Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.
Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3. -
疾病相关
Note=Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. -
序列相似性
Belongs to the kynureninase family. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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Functional Studies - Recombinant human L-Kynurenine Hydrolase protein (Active) (ab271581)
Specific activity of ab271581.
IC50 = 5.5 µM.
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SDS-PAGE - Recombinant human L-Kynurenine Hydrolase protein (Active) (ab271581)SDS-PAGE analysis of ab271581.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271581 尚未被引用在任何文献中。