重组人kynurenine 3-monooxygenase蛋白(Active) (ab271578)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 60% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人kynurenine 3-monooxygenase蛋白(Active)
参阅全部 kynurenine 3-monooxygenase 蛋白酶 -
生物活性
The specific activity of ab271578 was 100 pmol/min/μg.
ab271578 was tested with various amounts of enzyme. The reactions in KMO Assay Buffer (50 mM Sodium Phosphate, pH 7.5, 0.1% (w/v) Brij-35) contains 200 μM NADPH, 400 μM L-Kynurenine, and various concentrations of KMO. The reaction mixture incubated for 90 minutes while reading the UV absorption signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals at 340 nm in a plate reader.
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纯度
>= 60 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MDSSVIQRKK VAVIGGGLVG SLQACFLAKR NFQIDVYEAR EDTRVATFTR GRSINLALSH RGRQALKAVG LEDQIVSQGI PMRARMIHSL SGKKSAIPYG TKSQYILSVS RENLNKDLLT AAEKYPNVKM HFNHRLLKCN PEEGMITVLG SDKVPKDVTC DLIVGCDGAY STVRSHLMKK PRFDYSQQYI PHGYMELTIP PKNGDYAMEP NYLHIWPRNT FMMIALPNMN KSFTCTLFMP FEEFEKLLTS NDVVDFFQKY FPDAIPLIGE KLLVQDFFLL PAQPMISVKC SSFHFKSHCV LLGDAAHAIV PFFGQGMNAG FEDCLVFDEL MDKFSNDLSL CLPVFSRLRI PDDHAISDLS MYNYIEMRAH VNSSWFIFQK NMERFLHAIM PSTFIPLYTM VTFSRIRYHE AVQRWHWQKK VINKGLFFLG SLIAISSTYL LIHYMSPRSF LRLRRPWNWI AHFRNTTCFP AKAVDSLEQI SNLISR -
预测分子量
58 kDa including tags -
氨基酸
1 to 486 -
标签
His-DDDDK tag C-Terminus -
额外的序列信息
Genbank: NM_003679
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab271578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.01% TCEP, 20% Glycerol (glycerin, glycerine)
100 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- dJ317G22.1
- kmo
- KMO_HUMAN
see all -
功能
Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract. -
组织特异性
Highest levels in placenta and liver. Detectable in kidney. -
通路
Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3. -
序列相似性
Belongs to the aromatic-ring hydroxylase family. KMO subfamily. -
细胞定位
Mitochondrion outer membrane. - Information by UniProt
图片
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Functional Studies - Recombinant Human kynurenine 3-monooxygenase protein (Tagged) (ab271578)
Specific activity of ab271578 was 100 pmol/min/μg.
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SDS-PAGE - Recombinant Human kynurenine 3-monooxygenase protein (Tagged) (ab271578)SDS-PAGE analysis of ab271578.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271578 尚未被引用在任何文献中。