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AB268720

重组人KSR2蛋白(Tagged)

Recombinant Human KSR2 protein (Tagged)

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Recombinant Human KSR2 protein (Tagged) is a Human Fragment protein, in the 554 to 950 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE.

查看别名

Kinase suppressor of Ras 2, hKSR2, KSR2

1 Images
SDS-PAGE - Recombinant Human KSR2 protein (Tagged) (AB268720)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human KSR2 protein (Tagged) (AB268720)

SDS-PAGE analysis of ab268720.

关键信息

纯度

>80% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q6VAB6

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"RQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":950,"aminoAcidStart":554,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q6VAB6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The KSR2 protein also known as Kinase Suppressor of Ras 2 operates as a scaffold protein. It functions by assembling a protein complex that is important for signal transduction. KSR2 has a molecular weight of approximately 104 kDa. This protein is widely expressed in various tissues most notably in the brain and adipose tissue where it plays key roles in cellular communication and metabolic regulation.
Biological function summary

Kinase Suppressor of Ras 2 participates in the scaffolding of protein complexes required for MEK/ERK signaling. KSR2 contributes to the regulation of cellular energy homeostasis and metabolic processes. It also acts as a facilitator that enhances the signaling efficiency of the MAPK pathway allowing proper transduction of growth and developmental signals. Its role in the modulation of energy balance involves effects on both glucose and fatty acid metabolism.

Pathways

KSR2 interacts notably with the MEK/ERK and AMPK signaling pathways. KSR2 modulates the MAPK pathway facilitating the phosphorylation of ERK an essential step for transmitting signals from membrane receptors to the nucleus. In the AMPK pathway KSR2 interacts indirectly with AMPK-related proteins impacting energy balance and cellular stress responses. Through these pathways KSR2 links with key proteins like Raf MEK and LKB1 important for signal transduction and energy regulation.

KSR2 plays a significant role in obesity and type 2 diabetes. Mutations in KSR2 can lead to severe obesity accompanied by insulin resistance indicating its importance in regulating metabolism and energy consumption. Through these disorders KSR2 links with proteins such as AMPK which influences energy homeostasis and insulin signaling. Addressing KSR2 dysfunction may have therapeutic potential for metabolic diseases highlighting its importance in health and disease.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 (PubMed : 29433126). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

序列相似性

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

翻译后修饰

Phosphorylated on Ser-474 by MARK3.

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产品实验方案

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靶点信息

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 (PubMed : 29433126). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
See full target information Kinase suppressor of Ras 2
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搭配使用产品

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Primary Antibodies

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