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AB323152

Recombinant Human KMT4/Dot1L Protein Standard (His tag)

Recombinant Human KMT4/Dot1L Protein Standard (His tag)

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Recombinant Human KMT4/Dot1L Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.

查看别名

KIAA1814, KMT4, DOT1L, DOT1-like protein, Disruptor of telomeric silencing 1-like histone lysine methyltransferase, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4, H3-K79-HMTase

2 Images
Sandwich ELISA - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)

SDS-PAGE analysis of ab323152 under reducing conditions for 2ug protein.

关键信息

纯度

>80% SDS-PAGE

表达系统

Escherichia coli

标签

His tag C-Terminus

应用

sELISA, SDS-PAGE

applications

生物活性

No

访问

Q8TEK3

不含动物源

Yes

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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序列信息

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"49.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8TEK3","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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特殊说明

形式

Liquid

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常规信息

功能

Histone methyltransferase that methylates 'Lys-79' of histone H3 (PubMed : 12123582, PubMed : 38503750, PubMed : 38129415). Nucleosomes are preferred as substrate compared to free histones (PubMed : 12123582, PubMed : 38503750). Binds to DNA (PubMed : 12628190, PubMed : 38503750).

序列相似性

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Histone methyltransferase that methylates 'Lys-79' of histone H3 (PubMed : 12123582, PubMed : 38503750, PubMed : 38129415). Nucleosomes are preferred as substrate compared to free histones (PubMed : 12123582, PubMed : 38503750). Binds to DNA (PubMed : 12628190, PubMed : 38503750).
See full target information DOT1L
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