AB316571

重组人KLF4蛋白Standard (His tag)

Name: 重组人KLF4蛋白Standard (His tag) (ab316571)| Abcam中文官网
Brand: Abcam Limited
SKU: AB316571
Price: 1406 CNY
Availability: InStock

Recombinant Human KLF4 Protein Standard (His tag)

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Recombinant Human KLF4 Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.

查看别名

EZF, GKLF, KLF4, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor

2 Images

Sandwich ELISA - Recombinant Human KLF4 Protein Standard (His tag) (AB316571)

sELISA

Supplier Data

Sandwich ELISA - Recombinant Human KLF4 Protein Standard (His tag) (AB316571)

Sandwich ELISA with the capture antibody dilution at 2 ug/mL and detector antibody dilution at 0.5 ug/mL.

SDS-PAGE - Recombinant Human KLF4 Protein Standard (His tag) (AB316571)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human KLF4 Protein Standard (His tag) (AB316571)

SDS-PAGE analysis of ab316571

关键信息

纯度

>80% SDS-PAGE

表达系统

Escherichia coli

应用

SDS-PAGE, sELISA

applications

生物活性

访问

O43474

不含动物源

Yes

不含载体蛋白

种属

Human

存储溶液

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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序列信息

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"27.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O43474","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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性能和储存信息

运输条件

Dry Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

KLF4 also known as Kruppel-like factor 4 acts as a transcription factor involved in the regulation of gene expression. It has a molecular weight of approximately 55-60 kDa. Researchers often find KLF4 expressed in epithelial tissues such as the skin and gastrointestinal tract. It regulates cellular processes like differentiation proliferation and apoptosis playing a role in maintaining homeostasis and cell identity.

Biological function summary

KLF4 impacts cell differentiation and proliferation through its role as a transcription factor. It functions within several cellular complexes and interacts with other transcription factors to modulate gene expression. KLF4 regulates stem cell maintenance by influencing gene networks involved in self-renewal and differentiation contributing particularly to pluripotency in embryonic stem cells.

Pathways

The transcriptional activity of KLF4 integrates into key signaling pathways like the Wnt/β-catenin and Notch pathways. Within the Wnt/β-catenin pathway KLF4 may cooperate with β-catenin and TCF/LEF transcription factors to modulate gene targets. Similarly in the Notch signaling pathway KLF4 interacts with pathway components to influence cell fate decisions. These interactions highlight KLF4's central role in orchestrating complex signaling networks.

Mutations or dysregulated expression of KLF4 associates with various conditions including colorectal cancer and skin disorders like atopic dermatitis. In colorectal cancer KLF4 often interacts with other oncogenes and tumor suppressor proteins such as p53 to influence tumorigenesis. In skin disorders altered KLF4 expression can impact skin barrier function and inflammatory responses connecting it with proteins involved in immune response regulation.

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特殊说明

形式

Liquid

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常规信息

功能

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

序列相似性

Belongs to the krueppel C2H2-type zinc-finger protein family.

翻译后修饰

Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis. Ubiquitinated in a FBXO32-dependent manner, leading to proteasomal degradation.. Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6; deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis.

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information KLF4

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组人KLF4蛋白Standard (His tag)

Recombinant Human KLF4 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human KLF4 Protein Standard (His tag) (AB316571)

SDS-PAGE - Recombinant Human KLF4 Protein Standard (His tag) (AB316571)

关键信息

纯度

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液