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重组人KAT9 / Elp3蛋白(ab105858)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human KAT9 / Elp3 protein (ab105858)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 75% Densitometry
    • Suitable for: WB

    描述

    • 产品名称

      重组人KAT9 / Elp3蛋白

    • 纯度

      > 75 % Densitometry.
      ab105858 was determined to be >75% pure by densitometry. Affinity purified.

    • 表达系统

      Baculovirus infected Sf9 cells

    • Accession

      Q9H9T3

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 预测分子量

        86 kDa

      • 氨基酸

        1 to 547

    技术指标

    Our Abpromise guarantee covers the use of ab105858 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Western blot

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride

    常规信息

    • 别名

      • DKFZp761L098
      • Elongation protein 3 homolog
      • Elongation protein 3 homolog (S. cerevisiae)
      • ELONGATION PROTEIN 3, S. CEREVISIAE, HOMOLOG OF
      • elongator acetyltransferase complex subunit 3
      • Elongator complex protein 3
      • elp3
      • ELP3_HUMAN
      • FLJ10422
      • hELP3
      • Hypothetical protein DKFZp761L098
      • Kat9
      see all

    • 功能

      Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. May also have a methyltransferase activity.

    • 序列相似性

      Belongs to the ELP3 family.
      Contains 1 N-acetyltransferase domain.

    • 细胞定位

      Cytoplasm. Nucleus.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human KAT9 / Elp3 protein (ab105858)
      SDS-PAGE - Recombinant Human KAT9 / Elp3 protein (ab105858)
      ab105858 was determined to be >75% pure by densitometry.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab105858？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab105858 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    Is this purified protein fragment enzymatically active

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    Answer

    The lab have confirmed that under our own assay conditions, SIRT4 (ab101713) and KAT9(ELP3) (ab105858) proteins have not shown significant activity and that is why we have not labeled them as active. However, some phosphorylation may be present as we do not test specifically for phosphorylation, we only test for activity. SIRT4 is expressed in E.Coli and KAT9 is expressed in Sf9 cells so KAT9 is more likely to have some post translational modifications.

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