重组人KAT2B / PCAF蛋白(ab56275)
Key features and details
- Expression system: Escherichia coli
- Purity: > 70% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人KAT2B / PCAF蛋白
参阅全部 KAT2B / PCAF 蛋白酶 -
纯度
> 70 % Densitometry. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
69 kDa -
氨基酸
431 to 832 -
标签
GST tag N-Terminus -
额外的序列信息
Recombinant fragment, corresponding to amino acids 431-end of Human PCAF.
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技术指标
Our Abpromise guarantee covers the use of ab56275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
补充说明
PCAF is a binding protein that associates itself with p300/CBP and has no enzymatic activity. However storage at -80C and in glycerol is considered to prevent denaturation and to ensure that its binding conformation is conserved.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol, 0.87% Sodium chloride
常规信息
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别名
- CAF
- CREBBP associated factor
- GCN5
see all -
功能
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. -
组织特异性
Ubiquitously expressed but most abundant in heart and skeletal muscle. -
序列相似性
Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain. -
结构域
The bromodomain mediates binding to HIV-1 Tat. -
细胞定位
Nucleus. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human KAT2B / PCAF protein (ab56275)ab56275 on SDS-PAGE. The extra band found on the SDS gel may be a truncated form of PCAF, but because we have not yet done a Western Blot on this product, the identity is still unconfirmed.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab56275 尚未被引用在任何文献中。