重组人JHDM1D蛋白(Active) (ab271568)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人JHDM1D蛋白(Active) -
生物活性
Specific Activity: 0.2 pmole/min/μg. -
纯度
>= 90 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
AGAAAAVAAGAAAGAAAAAVSVAAPGRASAPPPPPPVYCVCRQPYDVNRF MIECDICKDWFHGSCVGVEEHHAVDIDLYHCPNCAVLHGSSLMKKRRNWH RHDYTEIDDGSKPVQAGTRTFIKELRSRVFPSADEIIIKMHGSQLTQRYL EKHGFDVPIMVPKLDDLGLRLPSPTFSVMDVERYVGGDKVIDVIDVARQA DSKMTLHNYVKYFMNPNRPKVLNVISLEFSDTKMSELVEVPDIAKKLSWV ENYWPDDSVFPKPFVQKYCLMGVQDSYTDFHIDFGGTSVWYHVLWGEKIF YLIKPTDENLARYESWSSSVTQSEVFFGDKVDKCYKCVVKQGHTLFVPTG WIHAVLTSQDCMAFGGNFLHNLNIGMQLRCYEMEKRLKTPDLFKFPFFEA ICWFVAKNLLETLKELREDGFQPQTYLVQGVKALHTALKLWMKKELVSEH AFEIPDNVRPGHLIKELSKVIRAIEEENGKPVKSQGI -
预测分子量
56 kDa -
氨基酸
2 to 488 -
标签
DDDDK tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab271568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
80 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- JHDM1D
- jmjc containing protein
- JmjC domain-containing histone demethylation protein 1D
see all -
功能
Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. -
序列相似性
Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger. -
结构域
The PHD-type zinc finger mediates the binding to H3K4me3. Binding to H3K4me3 prevents its access to H3K9me2.
The linker region is a critical determinant of demethylase specificity. It prevents the active site of JmjC to reach the target H3K9me2 when the PHD-type zinc finger binds to H3K4me3, while it favors selectivity toward H3K27me2. -
细胞定位
Nucleus. - Information by UniProt
图片
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Functional Studies - Recombinant human JHDM1D protein (Active) (ab271568)Specific activity of ab271568.
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SDS-PAGE - Recombinant human JHDM1D protein (Active) (ab271568)SDS-PAGE analysis of 7 µg ab271568.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271568 尚未被引用在任何文献中。