重组人Jagged1蛋白(Fc Chimera Active)

• FuncS

Supplier Data

Functional Studies - Recombinant human Jagged1 protein (Fc Chimera Active) (AB108575)

NIH/3T3 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin.

When the cells reached confluence, adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1μM, and 10μg/ml, respectively and continued for 2 days (day 0).

The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml of human Jagged1-Fc (ab108575) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7

• WB

Unknown

Western blot - Recombinant human Jagged1 protein (Fc Chimera Active) (AB108575)

Induction of Hes-1 with the treatment of recombinant Human Jagged1-Fc (ab108575).
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5μg/ml of ab108575 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

M : Marker
Lane 1 : ab108575, 0 min
Lane 2 : ab108575, 10 min
Lane 3 : ab108575, 30 min
Lane 4 : ab108575, 1 hr
Lane 5 : ab108575, 2 hr
Lane 6 : ab108575, 4 hr
Lane 7 : ab108575, 8 hr
Lane 8 : ab108575, 24 hr

All lanes:

Western blot - Recombinant human Jagged1 protein (Fc Chimera Active) (ab108575)

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