AB127522

重组人 IZUMO1 蛋白 (His-DHFR tag N-Terminus)

Name: 重组人IZUMO1蛋白 (ab127522)| Abcam中文官网
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SKU: AB127522
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Recombinant Human IZUMO1 protein (His-DHFR tag N-Terminus)

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(1 Publication)

Recombinant Human IZUMO1 protein (His-DHFR tag N-Terminus) is a Human Fragment protein, in the 51 to 211 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

查看别名

Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, Sperm-specific protein izumo, OBF, IZUMO1

关键信息

纯度

>95%

表达系统

Escherichia coli

应用

SDS-PAGE

applications

生物活性

访问

Q8IYV9

不含动物源

不含载体蛋白

种属

Human

复溶

Reconstitute in water

存储溶液

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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序列信息

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"18.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":211,"aminoAcidStart":51,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q8IYV9","tags":[{"tag":"His-DHFR","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

IZUMO1 also known as Immunoglobulin superfamily member 9 is a protein important to the process of fertilization. IZUMO1 has a molecular mass of approximately 37 kDa. It localizes specifically to the acrosomal region of sperm cells. This protein plays a mechanical role in sperm-egg fusion an essential step for successful fertilization allowing sperm cells to recognize and bind to the egg cell membrane. Expression of IZUMO1 is limited to mature sperm assuring its involvement exclusively during fertilization.

Biological function summary

The protein acts when spermatozoa interact with oocytes. IZUMO1 facilitates the binding through forming a complex with Juno the receptor on the egg surface. This interaction is necessary for the initial contact and fusion between the sperm and egg membranes. The IZUMO1-Juno pairing ensures proper docking and fusion enabling the combination of genetic materials from both gametes. The specificity of this binding prevents cross-species fertilization maintaining species integrity during reproduction.

Pathways

IZUMO1 has involvement in the fertilization pathway. It works directly in the sperm's fusion process an important part of the reproductive process. IZUMO1 interacts closely with other sperm proteins such as CD9 which play roles in adhesion and fusion events. These pathways incorporate IZUMO1 within the larger reproductive framework where it functions alongside these proteins to ensure successful gamete fusion and genetic transfer.

IZUMO1's functionality connects to issues of infertility. Defects or deficiencies in IZUMO1 expression or function can lead to male infertility due to ineffective sperm-egg fusion. Additionally fertility research identifies Juno as a complementary protein in this context as abnormalities in either protein may disrupt fertilization processes and contribute to reproductive challenges. Understanding IZUMO1 offers insight into diagnostic and therapeutic possibilities for treating infertility cases where sperm-egg membrane fusion is compromised.

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特殊说明

形式

Lyophilized

附加说明

>95% by SDS Page

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常规信息

功能

Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1 : IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human-specific oolemma epitope FCRL3/MAIA during fertilization (PubMed : 36070373). FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion (PubMed : 36070373). Plays a critical role in sperm-oolemma binding prior to plasma membrane fusion. Can mediate cell-cell fusion in cultured mammalian cells independently of its binding to IZUMO1R/JUNO (By similarity).

序列相似性

Belongs to the Izumo family.

翻译后修饰

N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm.. Phosphorylated. The cytoplasmic C-terminus is phosphorylated and undergoes phosphorylation changes during epididymal transit.

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information IZUMO1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 19:2234-2255 PubMed37151878

2023

Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Martinez,Donato Cappetta,Marialucia Telesca,Konrad Urbanek,Giuseppe Castaldo,Magali Dhellemmes,Vincenza Grazia Mele,Teresa Chioccarelli,Veronica Porreca,Anne-Laure Barbotin,Angèle Boursier,Florian Guillou,Charles Coutton,Sophie Brouillet,Antonella De Angelis,Liberato Berrino,Riccardo Pierantoni,Gilda Cobellis,Rosanna Chianese,Francesco Manfrevola

View all publications

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重组人 IZUMO1 蛋白 (His-DHFR tag N-Terminus)

Recombinant Human IZUMO1 protein (His-DHFR tag N-Terminus)

关键信息

纯度

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

复溶

存储溶液