重组人ITK/EMT蛋白(ab60300)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人ITK/EMT蛋白
参阅全部 ITK/EMT 蛋白酶 -
生物活性
Specific activity: 58 nmol/min/mg.
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纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
53 kDa -
氨基酸
352 to 620 -
标签
GST tag N-Terminus -
额外的序列信息
Recombinant fragment, corresponding to amino acids 352-end of Human ITK.
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
Previously labelled as ITK.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- EMT
- Homolog of mouse T cell itk/tsk
- IL 2 inducible T cell kinase
see all -
功能
Plays a role in T-cell proliferation and differentiation. -
组织特异性
T-cell lines and natural killer cell lines. -
疾病相关
Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
细胞定位
Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells. - Information by UniProt
图片
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Functional Studies - Recombinant human ITK/EMT protein (ab60300)The specific activity of ITK/EMT (ab60300) was determined to be 35 nmol/min/mg as per activity assay protocol and was equivalent to 50 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human ITK/EMT protein (ab60300)SDS PAGE analysis of ab60300 -
Functional Studies - Recombinant human ITK/EMT protein (ab60300)Sample Kinase assay plot. -
SDS-PAGE - Recombinant human ITK/EMT protein (ab60300)ab60300 on SDS-PAGE.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60300 尚未被引用在任何文献中。