重组人IRS1蛋白(ab71377)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人IRS1蛋白
参阅全部 IRS1 蛋白酶 -
纯度
> 90 % Densitometry. -
表达系统
Escherichia coli -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
氨基酸
1 to 355 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab71377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
常规信息
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别名
- HIRS 1
- HIRS1
- Insulin receptor substrate 1
see all -
功能
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
疾病相关
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
序列相似性
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
翻译后修饰
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human IRS1 protein (ab71377)SDS-PAGE showing ab71377 at approximately 65kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab71377 尚未被引用在任何文献中。