AB318948

重组人IRF5蛋白(His tag)

Name: 重组人IRF5蛋白(His tag) (ab318948)| Abcam中文官网
Brand: Abcam Limited
SKU: AB318948
Price: 2845 CNY
Availability: InStock

Recombinant Human IRF5 Protein (His tag)

Be the first to review this product! Submit a review

(0 Publication)

Recombinant Human IRF5 Protein (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >95%, 0.005 EU/µg endotoxin level, suitable for Mass Spec, SDS-PAGE.

查看别名

Interferon regulatory factor 5, IRF-5, IRF5

2 Images

Mass Spectrometry - Recombinant Human IRF5 Protein (His tag) (AB318948)

Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human IRF5 Protein (His tag) (AB318948)

Mass determination by ESI-TOF. Predicted MW is 57110 Da (+/- 10 Da by ESI-TOF). Observed MW is 57108.97 Da.

SDS-PAGE - Recombinant Human IRF5 Protein (His tag) (AB318948)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human IRF5 Protein (His tag) (AB318948)

SDS-PAGE analysis of ab318948 under reducing conditions for 2ug protein.

关键信息

纯度

>95% HPLC

内毒素水平

0.005 EU/µg

表达系统

Escherichia coli

应用

Mass Spec, SDS-PAGE

applications

生物活性

质谱

LC-MS/MS

访问

Q13568

不含动物源

Yes

不含载体蛋白

种属

Human

存储溶液

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.7293% Sodium chloride, 0.3002% Tris HCl, 0.0148% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

style

left-column

序列信息

[{"sequence":"MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLISPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNPIQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ","proteinLength":"Full Length","predictedMolecularWeight":"57.11 kDa","actualMolecularWeight":"57.12 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q13568","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

style

left-column

性能和储存信息

运输条件

Dry Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

IRF5 or Interferon Regulatory Factor 5 is a member of the IRF family of transcription factors. This protein weighs approximately 56 kDa and is expressed in cells of the immune system including B cells dendritic cells and macrophages. IRF5 plays an important role in regulating the transcription of interferon-stimulated genes following a viral infection. It acts by binding to specific DNA sequences initiating transcription processes that are important for the antiviral response.

Biological function summary

IRF5 influences the immune response through the regulation of cytokine production. It activates genes involved in producing pro-inflammatory cytokines such as TNF-α and IL-6 which are important for clearing viral infections and activating immune cells. IRF5 does not usually form part of a protein complex but it interacts closely with TRAF6 and MyD88 for its activation. Its pivotal role in cytokine production highlights its importance in controlling inflammation.

Pathways

IRF5 is an important component of the Toll-like receptor (TLR) signaling pathway and plays a role in the MyD88-dependent pathway. In this context IRF5 gets activated by TLRs interacting with MyD88 and TAK1 to enhance the transcription of important genes for immune response. It is also closely connected to the IRF3 protein which shares similar activation pathways and can similarly initiate an antiviral state.

Defects or dysregulation in IRF5 function are linked to autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE IRF5 influences autoantibody production contributing to disease development. It works alongside other regulatory proteins involved in autoimmunity including IFN-α and STAT4 which also play significant roles in modulating immune responses. Understanding IRF5's role in these conditions could present new opportunities for therapeutic interventions using IRF5 inhibitors.

style

left-column

特殊说明

形式

Liquid

style

left-column

常规信息

功能

Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed : 11303025, PubMed : 15695821, PubMed : 22412986, PubMed : 25326418, PubMed : 32433612). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (By similarity). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).

序列相似性

Belongs to the IRF family.

翻译后修饰

Phosphorylation of serine and threonine residues by IKBKB in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/EP300 and initiation of transcription.. 'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.

亚细胞定位

Nucleus

style

left-column

产品实验方案

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

See full target information IRF5

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组人IRF5蛋白(His tag)

Recombinant Human IRF5 Protein (His tag)

Mass Spectrometry - Recombinant Human IRF5 Protein (His tag) (AB318948)

SDS-PAGE - Recombinant Human IRF5 Protein (His tag) (AB318948)

关键信息

纯度

内毒素水平

表达系统

标签

应用

生物活性

质谱

访问

不含动物源

不含载体蛋白

种属

存储溶液