重组人Ionotropic Glutamate receptor 2蛋白Standard (His tag) (ab317299)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 80% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: Sandwich ELISA, SDS-PAGE
描述
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产品名称
重组人Ionotropic Glutamate receptor 2蛋白Standard (His tag) -
纯度
>= 80 % SDS-PAGE.
His tag affinity purified -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
Yes -
性质
Recombinant -
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种属
Human -
预测分子量
46 kDa including tags -
标签
His tag C-Terminus
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相关产品
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Corresponding Antibody
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Corresponding kit
技术指标
Our Abpromise guarantee covers the use of ab317299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Sandwich ELISA
SDS-PAGE
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形式
Liquid -
补充说明
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Information available upon request.
常规信息
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别名
- AMPA 2
- AMPA selective glutamate receptor 2
- AMPA-selective glutamate receptor 2
see all -
功能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily. -
翻译后修饰
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
细胞定位
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human Ionotropic Glutamate receptor 2 Protein Standard (His tag) (ab317299)
SDS-PAGE analysis of ab317299.
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Sandwich ELISA - Recombinant Human Ionotropic Glutamate receptor 2 Protein Standard (His tag) (ab317299)Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab317299 尚未被引用在任何文献中。
