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AB51240

重组人Interferon gamma蛋白

Recombinant Human Interferon gamma protein

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(2 Publications)

Recombinant Human Interferon gamma protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, WB, Biological Activity.

查看别名

Interferon gamma, IFN-gamma, Immune interferon, IFNG

3 Images
Sandwich ELISA - Recombinant Human Interferon gamma protein (AB51240)
  • sELISA

Unknown

Sandwich ELISA - Recombinant Human Interferon gamma protein (AB51240)

Standard curve for Interferon gamma (Analyte : ab51240); dilution range 1pg/ml to 1μg/ml using Capture Antibody ab25014 at 5μg/ml and Detector Antibody ab25101 at 0.5μg/ml.

Western blot - Recombinant Human Interferon gamma protein (AB51240)
  • WB

Unknown

Western blot - Recombinant Human Interferon gamma protein (AB51240)

All lanes:

Western blot - Anti-Interferon gamma antibody (<a href='/products/primary-antibodies/interferon-gamma-antibody-ab25101'>ab25101</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Interferon gamma protein (ab51240) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 19 kDa

true

Exposure time: 30s

SDS-PAGE - Recombinant Human Interferon gamma protein (AB51240)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Interferon gamma protein (AB51240)

ab51240 on 14% SDS-PAGE.

关键信息

纯度

>95% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

Escherichia coli

标签

Tag free

应用

WB, Biological Activity, SDS-PAGE

applications

生物活性

Yes

生物学活性

Measured in a cytotoxicity assay using WiDr cells. The ED50 for this effect is less or equal to 1.5 ng/ml.

访问

P01579

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab51240 can be used as a WB positive control in conjunction with <a href='/products/primary-antibodies/interferon-gamma-antibody-ab25101'>ab25101</a>.</p>" }, "Biological Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Measured in a cytotoxicity assay using WiDr cells. The ED50 for this effect is less or equal to 1.5 ng/ml.</p>" } } }
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您是否考虑以下替代产品？

AB259377

Recombinant Human Interferon gamma protein (Active)

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0 Reviews
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为什么推荐这个？

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序列信息

[{"sequence":"MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01579","tags":[]}]
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性能和储存信息

运输条件
Blue Ice
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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特殊说明

形式

Liquid

附加说明

Recombinant human Interferon gamma was expressed in *E.coli* and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

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常规信息

功能

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).

序列相似性

Belongs to the type II (or gamma) interferon family.

翻译后修饰

Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Chemical Society 145:20432-20441 PubMed37677157

2023

Strict Interactions of Fifth Letters, Hydrophobic Unnatural Bases, in XenoAptamers with Target Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Michiko Kimoto,Hui Pen Tan,Ken-Ichiro Matsunaga,Nur Afiqah Binte Mohd Mislan,Gota Kawai,Ichiro Hirao

Biologicals : journal of the International Association of Biological Standardization 45:52-60 PubMed27810255

2016

Is Pichia pastoris a realistic platform for industrial production of recombinant human interferon gamma?

Applications

Unspecified application

Species

Unspecified reactive species

Ali Razaghi,Emilyn Tan,Linda H L Lua,Leigh Owens,O P Karthikeyan,Kirsten Heimann
View all publications
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