重组人Interferon alpha 1蛋白(Active) (ab48750)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Sandwich ELISA
描述
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产品名称
重组人Interferon alpha 1蛋白(Active)
参阅全部 Interferon alpha 1 蛋白酶 -
生物活性
Interferon alpha 1 (ab48750) has the activity of 4 X 105 unit/mg (or 4 X 105 unit/ml).
This activity has been tested by evaluating anti-proliferation with Daudi cells. For Further information see Kim et al., 2009.
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纯度
> 95 % SDS-PAGE.
This protein was purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
CDLPETHSLD NRRTLMLLAQ MSRISPSSCL MDRHDFGFPQ EEFDGNQFQK APAISVLHEL IQQIFNLFTT KDSSAAWDED LLDKFCTELY QQLNDLEACV MQEERVGETP LMNADSILAV KKYFRRITLY LTEKKYSPCA WEVVRAEIMR SLSLSTNLQE RLRRKE -
预测分子量
20 kDa -
氨基酸
24 to 189 -
额外的序列信息
Full length mature protein, without the signal peptide.
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技术指标
Our Abpromise guarantee covers the use of ab48750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Sandwich ELISA
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形式
Liquid -
补充说明
Concentration determined by Bradford assay.
Endotoxin levels determined by LAL method.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- IFL
- IFN
- IFN alpha
see all -
功能
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
序列相似性
Belongs to the alpha/beta interferon family. -
细胞定位
Secreted. - Information by UniProt
图片
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Sandwich ELISA - Recombinant human Interferon alpha 1 protein (ab48750)
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SDS-PAGE - Recombinant human Interferon alpha 1 protein (ab48750)3ug of reduced ab48750 on SDS-PAGE, stained with Coomassie Blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab48750 被引用在 8 文献中.
- Constantin D et al. APOBEC3C, a nucleolar protein induced by genotoxins, is excluded from DNA damage sites. FEBS J 289:808-831 (2022). PubMed: 34528388
- Bencze D et al. Interactions between the NLRP3-Dependent IL-1β and the Type I Interferon Pathways in Human Plasmacytoid Dendritic Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36293012
- Guo C et al. Single-cell transcriptome profiling and chromatin accessibility reveal an exhausted regulatory CD4+ T cell subset in systemic lupus erythematosus. Cell Rep 41:111606 (2022). PubMed: 36351407
- Cotter KA et al. Mapping of m6A and Its Regulatory Targets in Prostate Cancer Reveals a METTL3-Low Induction of Therapy Resistance. Mol Cancer Res 19:1398-1411 (2021). PubMed: 34088870
- Lea AJ et al. Genome-wide quantification of the effects of DNA methylation on human gene regulation. Elife 7:N/A (2018). PubMed: 30575519
- Alagu J et al. Tumor Suppressor p14ARF Enhances IFN-?-Activated Immune Response by Inhibiting PIAS1 via SUMOylation. J Immunol 201:451-464 (2018). PubMed: 29848755
- Moriyama M et al. Two Conserved Amino Acids within the NSs of Severe Fever with Thrombocytopenia Syndrome Phlebovirus Are Essential for Anti-interferon Activity. J Virol 92:N/A (2018). PubMed: 30021900
- Sun B et al. Dengue virus activates cGAS through the release of mitochondrial DNA. Sci Rep 7:3594 (2017). PubMed: 28620207