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重组人immunodeficiency virus HIV-2 Protease蛋白(ab113857)

Submit a review Q&A (1)
SDS-PAGE - Recombinant human immunodeficiency virus HIV-2 Protease protein (ab113857)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Suitable for: Inhibition Assay, Functional Studies

    描述

    • 产品名称

      重组人immunodeficiency virus HIV-2 Protease蛋白

    • 生物活性

      Kinetic parameters: Km= 740 µM Kcat= 30 s-1 Kcat/Km= 4.1mM-1s-1 with peptide substrate ATLNFPISPW

    • 纯度

      > 95 % SDS-PAGE.
      Purity as determined by densitometric image analysis: >95%

    • 表达系统

      Escherichia coli

    • Accession

      AAT99495

    • 蛋白长度

      Protein fragment

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human immunodeficiency virus

      • 预测分子量

        11 kDa

      • 氨基酸

        1 to 99

    技术指标

    Our Abpromise guarantee covers the use of ab113857 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Inhibition Assay

      Functional Studies

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.00
      Constituents: 0.39% MES, 0.05% Polyethylene glycol, 0.008% DTT, 0.24% Tris, 0.03% EDTA, 10% Glycerol (glycerin, glycerine), 1.17% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    图片

    • SDS-PAGE - Recombinant human immunodeficiency virus HIV-2 Protease protein (ab113857)
      SDS-PAGE - Recombinant human immunodeficiency virus HIV-2 Protease protein (ab113857)
      analysis of ab113857 by SDS-PAGE

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab113857？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab113857 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    Phone call requesting information on if all the HIV protease proteins (ab113857, ab124313, ab124858 and ab84117) are active or not.

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    Answer

    Thank you for contacting us yesterday and you interest in our products.

    I have had a look into your query relating to whether the HIV protease proteins (ab113857, ab124313, ab124858 and ab84117) are all active. The title you have noticed where "Active" is included is down to an inconsistency in naming in our catalogue, it is not an indicator that ab113857 or ab124858 are not active. I am sorry for this confusion, I am going to try to have this amended.

    Each one of the proteins have been shown to have enzyme activity through peptide cleavage assays. Details of these assays can be found on each of the individual datasheets of the products. This means that all of them should be suitable for your experiment if you are using a peptide sequence recognised by the protease. The experiment you are hoping to perform would be covered by the Abpromise guarantee. More information on this guarantee can be found from the following page:

    https://www.abcam.com/abpromise

    If you would like to try any of the proteins, I'd like to encourage you tosubmit anAbreview via the online product datasheet once you have used it in your assay. As a thank you you will be rewarded with Abpoints which can be used to redeem against Amazon vouchers or future purchases from us. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com