重组人ILF3蛋白(ab132543)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE, ELISA
描述
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产品名称
重组人ILF3蛋白 -
表达系统
Wheat germ -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWID EQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAK GLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSV DDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDR QKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWP LELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEAT DAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNE NPVDYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKAEPP QAMNALMRLNQLKPGLQYKLVSQTGPVHAPIFTMSVEVDGNSFEASGPSK KTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEETEAKPAVVAPAPVVE AVSTPSAAFPSDATAEQGPILTKHGKNPVMELNEKRRGLKYELISETGGS HDKRFVMEVEVDGQKFQGAGSNKKVAKAYAALAALEKLFPDTPLALDANK KKRAPVPVRGGPKFAAKPHNPGFGMGGPMHNEVPPPPNLRGRGRGGSIRG RGRGRGFGGANHGGYMNAGAGYGSYGYGGNSATAGYSDFFTDCYGYHDFG SS -
预测分子量
103 kDa including tags -
氨基酸
1 to 702 -
标签
GST tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab132543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
ELISA
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形式
Liquid -
补充说明
This product was previously labelled as ILF3.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
常规信息
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别名
- CBTF
- Double stranded RNA binding protein 76
- Double-stranded RNA-binding protein 76
see all -
功能
May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. -
组织特异性
Ubiquitous. -
序列相似性
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 DZF domain. -
翻译后修饰
Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
Methylated by protein arginine N-methyltransferase 1.
Arg-609 is dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab132543 被引用在 1 文献中.
- Nussbacher JK & Yeo GW Systematic Discovery of RNA Binding Proteins that Regulate MicroRNA Levels. Mol Cell 69:1005-1016.e7 (2018). PubMed: 29547715