JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB316645

重组人IL-4R蛋白Standard (His tag)

Recombinant Human IL-4R Protein Standard (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human IL-4R Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.

查看别名

CD124, IL4RA, 582J2.1, IL4R, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA

2 Images
Sandwich ELISA - Recombinant Human IL-4R Protein Standard (His tag) (AB316645)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human IL-4R Protein Standard (His tag) (AB316645)

Sandwich ELISA with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.

SDS-PAGE - Recombinant Human IL-4R Protein Standard (His tag) (AB316645)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human IL-4R Protein Standard (His tag) (AB316645)

SDS-PAGE analysis of ab316645 under reducing conditions for 2ug protein

关键信息

纯度

>80% SDS-PAGE

表达系统

HEK 293 cells

标签

His tag C-Terminus

应用

SDS-PAGE, sELISA

applications

生物活性

No

访问

P24394

不含动物源

Yes

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

style
left-column

序列信息

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"25.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P24394","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

特殊说明

形式

Liquid

style
left-column

常规信息

功能

Receptor for both interleukin 4 and interleukin 13 (PubMed : 17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.

序列相似性

Belongs to the type I cytokine receptor family. Type 4 subfamily.

翻译后修饰

On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.. The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.

style
left-column

产品实验方案

style
left-column

靶点信息

Receptor for both interleukin 4 and interleukin 13 (PubMed : 17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
See full target information IL4R
style
left-column
style
left-column
style
right-column

搭配使用产品

Proteins & Peptides

AB259406

Recombinant mouse IL-4 protein (Active)

Premium Bioactive

Functional Studies - Recombinant mouse IL-4 protein (Active) (AB259406)

  • 0
  • 0
Primary Antibodies

AB259498

Anti-IL-4R antibody [EPR21947-278] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-4R antibody [EPR21947-278] - BSA and Azide free (Detector) (AB259498)

  • 0
  • 0
Proteins & Peptides

AB307475

Recombinant Human CD86 protein (Fc Chimera)

Mass Spectrometry - Recombinant Human CD86 protein (Fc Chimera) (AB307475)

  • 0
  • 0
Proteins & Peptides

AB259402

Recombinant human IL-10 protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human IL-10 protein (Active) (AB259402)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com