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AB179620

重组人IL-4蛋白(Animal Free)

Recombinant human IL-4 protein (Animal Free)

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Recombinant human IL-4 protein (Animal Free) is a Human Full Length protein, in the 25 to 153 aa range, expressed in Escherichia coli, with >98%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, FuncS.

查看别名

Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4

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Cellular Activation - Recombinant human IL-4 protein (Animal Free) (AB179620)
  • CellAct

Supplier Data

Cellular Activation - Recombinant human IL-4 protein (Animal Free) (AB179620)

Dose-dependent proliferation of TF-1 cells using ab179620. Cells were incubated for 12 hours and then measured at 490 nm. Assays were carried out in triplicate.

关键信息

纯度

>98% SDS-PAGE

内毒素水平

<1 EU/µg

表达系统

Escherichia coli

标签

Tag free

应用

FuncS, SDS-PAGE, HPLC

applications

生物活性

Yes

生物学活性

The activity is determined by a proliferation assay using MC-9 cells and is typically less than 0.2 ng/mL.

访问

P05112

不含动物源

Yes

不含载体蛋白

No

种属

Human

复溶

Reconstitute at 0.1 mg/mL in water

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

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序列信息

[{"sequence":"MHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS","proteinLength":"Full Length","predictedMolecularWeight":"15 kDa","actualMolecularWeight":null,"aminoAcidEnd":153,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P05112","tags":[]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
True
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特殊说明

形式

Lyophilized

附加说明

Protein content and purity determined by HPLC, reducing and non-reducing SDS-PAGE, UV spectroscopy at 280 nm.

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常规信息

功能

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).

序列相似性

Belongs to the IL-4/IL-13 family.

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产品实验方案

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靶点信息

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).
See full target information IL4
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详情请参阅我们的条款与条件。
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