重组人IL-4蛋白(ab119465)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: WB, ELISA, Functional Studies, SDS-PAGE, HPLC
描述
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产品名称
重组人IL-4蛋白
参阅全部 IL-4 蛋白酶 -
生物活性
The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is = 0.2 ng/ml, corresponding to a specific activity of = 5 x 106 units/mg. -
纯度
> 98 % SDS-PAGE.
Greater than 98% by HPLC -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAAT VLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCP VKEANQSTLENFLERLKTIMREKYSKCSS -
预测分子量
15 kDa -
氨基酸
25 to 153 -
额外的序列信息
Full length mature chain without signal peptide.
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技术指标
Our Abpromise guarantee covers the use of ab119465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
ELISA
Functional Studies
SDS-PAGE
HPLC
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Buffer prior to lyophilisation is water with trace amounts of 0.1% TCA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with 50ul distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. It is recommended that the vial is gently mixed after reconstitution.
常规信息
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别名
- B cell growth factor 1
- B cell IgG differentiation factor
- B Cell Stimulatory Factor 1
see all -
功能
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
疾病相关
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
序列相似性
Belongs to the IL-4/IL-13 family. -
细胞定位
Secreted. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab119465 尚未被引用在任何文献中。
